Fig. 1

Centriolar proteins localize to the DC (group v). a The current dogma is that mature sperm have one centriole. The spermatid neck has a PC and DC, and the ejaculated spermatozoon has a PC and an empty space, the vault, which marks the location of the degenerated DC (Manandhar et al.⁶). b Graphical summary of the groups of the localization patterns of proteins in the sperm. Group i is centriolar proteins that were localized to the PC area only and is consistent with the dogma. Group ii is PCM proteins that were absent from the sperm, as is consistent with the dogma. Group iii are PCM proteins that were unexpectedly localized to the striated columns or capitulum. Group iv are centriolar proteins that were unexpectedly localized to the striated columns or capitulum, or were unexpectedly absent altogether. Group v are proteins that were unexpectedly present in the DC and the PC, which are inconsistent with the dogma. Group v is shown in c–f. c Antibodies against the centriole tip proteins CETN1/2 and POC5 labeled the PC and DC. d Antibodies against CEP63 and CPAP labeled near the CETN1/2 or POC5-labeled PC and DC. e Antibodies against CEP290 labeled adjacent to the CETN1/2-labeled DC, presumably marking the junction of the DC with the axoneme. f Three distinct antibodies against POC1B labeled both the PC and DC. Unlike other centriolar proteins, POC1B was enriched in the DC relative to the PC (POC1B-P: 2.1 ± 0.5, POC1B-537: 2.74 ± 1.64, POC1B-5G5A7: 1.92 ± 0.41, N > 6). POC1B-P, Rabbit polyclonal antibody from the Pearson lab; POC1B-537, Rabbit polyclonal antibody from the Avidor-Reiss lab; POC1B-5G5A7 Rat monoclonal Ab from the Avidor-Reiss lab. N nucleus, ne sperm neck, t tail; scale bars 1 μm