Table 2 The phenotypes studied in this work
Phenotype | Selective condition | Control condition |
|---|---|---|
Essentiality | dCas9, LB | Empty plasmid, LB |
Auxotrophy | MOPS | LB |
L-Trp biosynthesis | 0.5 g/L casamino acid, MOPS | LB |
Furfural tolerance | 0.4 g/L furfural, MOPS | initial, see Supplementary Fig. 7 |
Isobutanol tolerance | 4 g/L isobutanol, MOPS | initial, see Supplementary Fig. 7 |
