Fig. 7

MiR-103/lncWDR59 conserved function in vivo during atherosclerosis. Apoe^−/− mice were fed 12 weeks of HFD and were injected four times (once per week) with TSBs or control-LNA oligonucleotides (0.5 mg per 20 g). Paxgene-fixed/paraffin-embedded roots were sectioned and used for (a) elastic van Gieson (EVG) staining, (b) Ki67, and (c) γH2AX immunofluorescence staining. a EVG-stained aortic roots were used to quantify the plaque area per valve, the necrotic core area (expressed as percentage of total plaque area), and the fibrous cap thickness (n = 5 mice per group, 3–4 slides per mouse). Scale bar: 200 µm. b,c The number of Ki67⁺/ and γH2AX⁺/vWF⁺ cells was divided to the total number of vWF⁺ cells and expressed as percentage (n = 5 mice per group, 3 slides per group). Ctrl control-LNA, TSB target site blockers. *P < 0.05 and **P < 0.01 by Student’s t-test. Scale bar: 25 µm