Table 1 Haplotypes of pfcrt-edited and control P. falciparum lines

From: Emerging Southeast Asian PfCRT mutations confer Plasmodium falciparum resistance to the first-line antimalarial piperaquine

  

PfCRT haplotype at listed position

Parasite line

Edited pfcrt

72

74

75

76

97

144

145

148

194

220

271

326

333

343

353

356

371

Dd2

No

C

I

E

T

H

A

F

L

I

S

E

S

T

M

G

T

I

Dd2Dd2 crt

Yes

C

I

E

T

H

A

F

L

I

S

E

S

T

M

G

T

I

Dd2Dd2 crt F145I

Yes

C

I

E

T

H

A

I

L

I

S

E

S

T

M

G

T

I

Dd2Dd2 crt M343L

Yes

C

I

E

T

H

A

F

L

I

S

E

S

T

L

G

T

I

Dd2Dd2 crt G353V

Yes

C

I

E

T

H

A

F

L

I

S

E

S

T

M

V

T

I

PH1008-C

No

C

I

E

T

H

A

F

L

I

S

E

S

T

L

G

T

I

PH1008-CDd2 crt

Yes

C

I

E

T

H

A

F

L

I

S

E

S

T

M

G

T

I

PH1263-C

No

C

I

E

T

Y

A

F

L

I

S

E

S

T

M

G

T

I

PH1263-CDd2 crt

Yes

C

I

E

T

H

A

F

L

I

S

E

S

T

M

G

T

I

Cam734

No

C

I

D

T

H

F

F

I

T

S

E

N

S

M

G

I

R

GB4

No

C

I

E

T

H

A

F

L

I

S

E

N

T

M

G

I

I

3D7

No

C

M

N

K

H

A

F

L

I

A

Q

N

T

M

G

I

R

  1. pfcrt was edited using customized zinc finger-nucleases, and clones were generated by limiting dilution. Differences from the 3D7 wild-type allele are shown in italics for Dd2 and bold for Cam734 mutations. Underline indicates mutations identified from piperaquine-resistant field isolates and tested herein by gene editing
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