Fig. 4

In vivo infiltration is highly impaired in TEAD1KO GBM xenografts. a Representative immunofluorescent histology of tumor engraftment and core growth near the injection site in sham and TEAD1KO cells, 3.5 months after orthotopic xenotransplantation. b Representative immunofluorescence images of TEAD1 expression, confirming the stable loss of TEAD1 in TEAD1KO xenografts. Insets represent the entire TEAD1 immunofluorescence image with DAPI co-labeling. c Quantification of cell proliferation in sham and TEAD1KO cells at 3.5 months post xenotransplantation (n = 3 animals per condition; Ki67+ cells counted out of all HNA+ cells in 11 serial histological sections. Bars represent mean ± SEM). d Quantification of migratory tumor spread in sham and TEAD1KO cells at 3.5 months post xenotransplantation (n = 3 animals per condition; ***p = 0.0003 for both. Area = sum of areas with tumor spread in 11 serial coronal sections. Volume = average area × the distance between the first and the last serial section examined. Bars represent mean ± SEM). Schematic of the analyzed mouse brain serial coronal sections is depicted on the right. e Representative example of infiltrative tumor spread along the corpus callosum in sham and TEAD1KO cells at 3.5 months post xenotransplantation. HNA: human nuclear antigen, CC: corpus callosum, v: ventricle, a anterior, p: posterior. Scale bar = 50 μM