Fig. 7

PD-1⁺ CD8⁺ TILs from breast tumors retain cytotoxic capacity. a Non-naive CD8⁺ T cells isolated from bcPBMCs or bcTumor were cultured with CD19 expressing target cells and CD3-CD19 bi-specific antibodies and analyzed for CD107 mobilization by flow cytometry as shown in representative plots. b Graph depicts change (Δ) in frequency of CD107 mobilizing CD8⁺ cells in co-cultures with bi-specific antibody relative to those without. Parent gates were of PD-1⁺ and PD-1⁻ populations from bcPBMC and bcTumor CD8⁺ CD45RA⁻ T cells. c Graph depicts cytotoxicity of bcPBMC and bcTumor CD8⁺ after overnight co-culture with bi-specific antibodies and target cells. Significance was calculated using one-way ANOVA and Holm–Sidak multiple comparison tests; *p < 0.05; **p < 0.01