Fig. 5 | Nature Communications

Fig. 5

From: High-throughput three-dimensional chemotactic assays reveal steepness-dependent complexity in neuronal sensation to molecular gradients

Fig. 5

STK11 affects Sema3A-induced chemorepellence of neurite outgrowth. a Fluorescence images of neurons in a Sema3A gradient of various steepness after STK11 knockdown. Scale bar: 100 µm. b Quantitative analysis of STK11’s role in neuronal migration and related association with Sema3A gradient steepness, n = 3, error bars indicate standard deviation. c Box-plots for quantitative analysis of STK11’s role in the chemorepellent guidance of neurite outgrowth in response to varied Sema3A gradient steepness. The parts of the box indicate 25, 50, and 75 percentiles, and the whiskers indicate 5% and 95%. The square mark indicates mean of the data. More than 20 neurites (as indicated on top of each box) were pooled from three biological replicates. For b, c, the red line indicates logarithmic fitting of the data mean; neuronal growth pattern in each hydrogel cylinder was compared in pairwise to experiments with regular Sema3A treatment (SMA-norm), * indicates a p-value < 0.05 by paired Kruskal–Wallis tests

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