Fig. 7
Summary of neuronal sensitivity to the steepness of Sema3A gradient. a Cell migration and b neurite repellence under different conditions, including Sema3A only (red, square), Sema3A plus STK11 knockdown (STK−, black, triangle), or Sema3A plus GSK3 inhibition (GSK3−, blue, circle). The solid lines indicate the logarithmic fitting of the data point. Data were quantified from at least three biological replicates. The association of neuronal growth (neurite guidance or soma migration) with the steepness of chemotactic gradient was examined using a linear fit for the neuronal growth parameters and gradient steepness variation. A p-value was calculated for the regression coefficient (gradient steepness), which, in a linear regression model, is based on a t-test with a null hypothesis that the coefficient is zero, and p < 1 × 10−2 indicates a significant association. The summary of p-values is included in Supplementary Table 1
