Fig. 1 | Nature Communications

Fig. 1

From: Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease

Fig. 1

Identification of tissue-of-origin of cfDNA using deconvolution of the plasma methylome aided by a comprehensive methylation atlas. a Methylation atlas composed of 25 tissues and cell types (columns) across ~8000 CpGs (rows). For each cell type, we selected the top 100 uniquely hypermethylated (top) and 100 most hypomethylated (bottom) CpG sites, giving a total of 5000 tissue-discriminating individual CpGs. We then added neighboring (up to 50 bp) CpGs, as well as 500 CpGs that are differentially methylated across pairs of otherwise similar tissues. Overall, we used 7890 CpGs that are located in 4039 500 bp genomic blocks. b Deconvolution of plasma DNA. Cell-free DNA (cfDNA) is extracted from plasma and analyzed with a methylation array. It is then deconvoluted using a reference methylation atlas to quantify the contribution of each cell type to the cfDNA sample

Back to article page