Fig. 3
Enzymatic parameters of FANA enzymes. a, b Pre-steady state kinetic plots of NGS12-1 and NGS12-7 in buffer (pH 8.5) containing 25 mM MgCl2 and 200 mM NaCl at 24 °C ([Fz] = 2.5 µM, [S] = 0.5 µM; n = 3; error bars represent standard deviation, s.d.). c, d pH and Mg2+ activity profile of NGS12-7 at 24 °C. e Representative gel of NGS12-7 showing RNA cleavage activity under multiple-turnover conditions at pH 8.5, 24 °C ([Fz] = 10 nM, [S] = 0.5 µM). f Michaelis–Menten kinetic profile of NGS12-7. n = 3; R2 ≥0.95
