Fig. 1 | Nature Communications

Fig. 1

From: Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification

Fig. 1

Gauss vs. flat-top illumination profiles. a Traditional illumination profile for TIRF microscopy with a Gaussian laser beam (left) compared to a flat-top profile created by a refractive beam-shaping device in the excitation path (right). b Line plots of fluorescence intensity along x and y axes (red and blue, respectively) for both profiles in a. Scale bars, 20 µm in a

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