Fig. 5

hmcn-1 is required for maintaining parenchymal differentiated cell localization. a Confocal z-projections from the red rectangle as indicated in the diagrams (upper panel) show ectopic localization of cells expressing markers of differentiated cell types (Methods) outside of the 6G10⁺ muscle layer at 20 days of RNAi. b Confocal slice through side of the animal as diagrammed to the left shows ectopic localization of a smedwi-1⁺ neoblast and smedwi-1⁻ cells with non-epidermal nuclear morphology outside the parenchyma near a hole in the muscle layer at 20 days of RNAi. Anterior, left, four independent experiments. c Animals were fed with dsRNA (F) as indicated in top timeline and lethally irradiated at 12 days of RNAi. In the absence of neoblasts at 23 days of RNAi, epidermal ruffling was readily apparent (left image: scale bar, 100 μm) and collagen⁺ nuclei were found outside of the muscle layer, quantified in graph below. Number of nuclei outside of ventral muscle fibers normalized by animal area is plotted with mean, standard deviation, and p-value from Kruskal–Wallis test with Dunn’s correction. d Diagram summarizing the defects after inhibition of hmcn-1. Ep, epidermis. Pa, parenchyma. White arrowhead, ectopic cells. Unless otherwise specified, fractions indicate number of total animals from 2 independent RNAi experiments with phenotype shown in representative image, anterior is up, scale bars, 10 μm