Fig. 1
Olaparib induces a senescence-like phenotype in HGSOC cell lines. a Cell proliferation curves of HGSOC H2B-GFP cell lines exposed to increasing concentrations of Olaparib. b, c HGSOC dead cells analyzed by flow cytometry (b) and SAβgal positive HGSOC cells (c) following 6 days treatment with selected Olaparib concentrations (Supplementary Fig. 1A). d HGSOC cell morphology analyzed by flow cytometry following 6 days of treatment with Olaparib IC50 concentrations (see Supplementary Fig. 1A, E for details). e, f Levels of IL-6 (e), IL-8 (f) were measured by ELISA assay following 6 days treatment with Olaparib IC50 concentrations. g Number of γ-H2AX foci per nucleus in HGSOC cells lines following 6 days of treatment with Olaparib IC50 concentrations. h, i Analysis of 8-h (h) or 24-h (i) EdU pulse after 6 days exposure of HGSOC cells to Olaparib IC50 concentrations. j Flow cytometry analysis of cell cycle populations following 6 days exposure of HGSOC cells to Olaparib IC50 concentrations. Data in (a) are representative curves of at least three independent experiments. For all the data, the mean ± SEM of three independent experiments is shown. Data were analyzed using the two-tail Student t test. *Denotes p < 0.05, **p < 0.01, and ***p < 0.001
