Fig. 2 | Nature Communications

Fig. 2

From: Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex

Fig. 2

VEX-complex compartmentalisation is transcription dependent. a, b Protein-blot and immunofluorescence analysis of VEX1myc (a) and mycVEX2 (b) before and after BMH-21 treatment (30 min at 1 μM). Proportions of nuclei displaying 1–2 major foci or distributed signals are indicated; the remaining cells displayed no detectable signal. Values are averages of two independent experiments (≥100 nuclei each). Error bars, SD; **p < 0.01; ***p < 0.001 (two-tailed paired Student’s t test). DNA was counter-stained with DAPI; the images correspond to maximal 3D projections of 0.1 μm stacks; scale bars 2 μm; the data are representative of independent biological replicates and independent experiments. Source data are provided as a Source Data file

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