Fig. 8 | Nature Communications

Fig. 8

From: Monoallelic expression and epigenetic inheritance sustained by a Trypanosoma brucei variant surface glycoprotein exclusion complex

Fig. 8

Colocalisation of CAF-1 and the VEX-complex during S phase. a, b Immunofluorescence-based colocalisation studies of VEX1myc/CAF-1bGFP and VEX2GFP/CAF-1bmyc. Representative images from G1 and S phase cells are shown. Nuclei (n ≥ 70) were scored for CAF-1b puncta coincident with VEX1myc/VEX2GFP foci. DNA was counter-stained with DAPI; scale bars, 2 μm. Error bars, SD; *p < 0.05; **p < 0.01; ***p < 0.001 (one-way ANOVA test). Values are averages from two independent biological replicates and are representative of independent experiments. The images on the right-hand side correspond to maximal 3D projections of 0.1 μm stacks. c Co-immunoprecipitation of VEX2GFP/CAF-1bmyc followed by protein-blot analysis. Green circle, GFP; black circles, myc; I, input; E, elution. Source data are provided as a Source Data file

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