Fig. 7

FIRE deletion results in selective loss of microglia. a Cryosections of adult brains were stained with an antibody against IBA1. Boxed area shows an IBA1⁺ perivascular macrophage in the Csf1r^{ΔFIRE/ΔFIRE} mouse brain. Scale bars = 100 µm, 50 µm in the upper and lower panels, respectively. Images are representative of four mice per genotype. b Cryosections of adult brains were stained with an antibody against P2RY12. Scale bar = 40 µm. c Single cell suspensions of myelin-depleted brains from males and females aged 10 weeks to 9 months were analyzed by flow cytometry for CD45 and CD11b expression as per⁴⁵. Microglia = CD45^lowCD11b⁺. Macrophages = CD45⁺CD11b⁺. n = 8+/+, 5+/Δ, 8 Δ/Δ from five experiments. P < 0.0001 (****) Δ/Δ compared to +/+. d Cryosections of adult brains stained with an antibody against IBA1 using the same cohort of mice in a and b. Upper panel shows the choroid plexus (CP) and white arrows point to perivascular macrophages in the lower panel. Images are representative of three mice. Scale bars = 40 and 20 µm in the upper and lower panels, respectively. e Dissected meningeal dura mater was stained with antibodies against IBA1 and CD169. Images are representative of two mice per genotype. Scale bars = 50 µm. All source data are provided within a Source Data excel file. Graphs show mean + SEM and P values were determined by two-tailed t-tests