Fig. 5 | Nature Communications

Fig. 5

From: The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model

Fig. 5

The double-mutant R480A-Q482A abolishes Fe3+-enterobactin binding. a Unlike the wild-type protein (blue line), the double-mutant R480A-Q482A (red line) does not coelute with Fe3+-enterobactin. Proteins were incubated with the Fe3+-siderophore before being loaded on a S200 size-exclusion column. UV-visible spectra of PfeA shows a peak of absorption around 550 nm characteristic of the iron-siderophore complex. b Isothermal calorimetry titration of Fe3+-enterobactin with PfeA (blue) and mutants R480A-Q482A (red), G324V (cyan), R480A (green) and Q482A (black)

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