Fig. 1
From: CENP-A nucleosome clusters form rosette-like structures around HJURP during G1
SMLM images of centromeres in a non-synchronized U2OS cell. a Conventional epifluorescence image of CENP-A. b High-resolution SMLM image of the same region revealing that CENP-A in fact forms characteristic clusters. c Zoomed view of several centromeres from this cell. d Cluster #4 imaged in classical epifluorescence microscopy (left), SMLM (center) and represented as a Voronoi diagram built on localizations of fluorophores (right). e Larger region of the Voronoi diagram containing cluster #4 and other clusters. A significant portion of the non-synchronized cells have centromeres of this rosette-like shape, suggesting that they are formed in a particular phase of the cell cycle. Scale bars, 500 nm (a, b, e) and 100 nm (c, d); for similar experiments in HeLa cells see Supplementary Figs. 1 and 2
