Fig. 1

Single-cell transcriptomic analysis reveals human retina diversity. a Study design and sample preparation. Postmortem human retinas were enzymatically dissociated and single-cells were isolated. cDNA single-cell libraries were generated and sequenced. We profiled 20,091 cells across the retinas of three normal individuals using a droplet-based microfluidics scRNA-seq platform. b Sketch of retina cross-section showing layers and major cell types. c Cell-to-cell similarity network of retinal cells. d Average expression of known cell-type marker genes across cell groups. e Projection of known cell-type-specific marker gene expression across cell groups. d, e show localization of distinct cell types within the network, identifying neighborhoods of rods, cones, retinal ganglion cells, bipolar cells, amacrine cells, horizontal cells, macroglia (Müller glia and astrocytes), microglia, and vascular cells