Fig. 5
From: High-performance chemical- and light-inducible recombinases in mammalian cells and mice
Applications of split recombinases. a A genetic switchboard using three inducible split recombinases to individually control expression of three therapeutically relevant human cytokines, interferon-γ (IFNγ), interleukin-2 (IL-2), and interleukin-10 (IL-10). Drugs were added 1 day post transfection. b Measurement of supernatant cytokine concentration using enzyme-linked immunosorbent assays (n = 3). c A xenograft mouse model of split recombinases used in a that were transfected in human embryonic kidney (HEK) cells alongside a corresponding luciferase reporter that expresses upon site-specific recombination. Transfected cells are transplanted subcutaneously (s.c.) and drugs or vehicle-only control are injected intraperitoneally (i.p.). d Time-course depicting time of transfection, injections, and imaging. e Images and averaged values of emitted luminescence from three split-recombinase systems. Error bars of mean cytokine and luminescence values indicate the arithmetic standard error of the mean. Source data are provided as a Source Data file
