Fig. 4

The lineage identities of cyst epithelial cells. a Schematic diagram showing nephron segments positive for each marker. “C” indicates the cortex, “OM” indicates the outer medulla, and “IM” indicates the inner medulla. b Expressions of nephron segment markers in a severe-type kidney. Small boxes indicate regions containing representative cysts positive for AQP1, AQP2, NCC, or UMOD, respectively; cyst areas that are positive for each marker are surrounded by white lines. Scale bar in large images, 1 mm. Scale bar in small boxes, 100 µm. c Box plot of the areas of AQP1-, AQP2-, NCC-, or UMOD-positive cysts in three severe-type kidneys. n = 768 cysts in three animals. d Expressions of nephron segment markers in an intermediate-type kidney. Small boxes indicate the regions shown at high magnification in f. Cyst areas that are positive for AQP1, AQP2, or UMOD, respectively, are surrounded by white lines. ND indicates not detected. Scale bar, 1 mm. e Box plot of the areas of AQP1-, AQP2-, NCC-, or UMOD-positive cysts in the intermediate-type kidneys. “C” indicates the cortex, “OM” indicates the outer medulla, “IM” indicates the inner medulla. n = 1008 cysts in one animal. f Expressions of nephron segment markers in an intermediate-type kidney compartment containing multiple cysts similar to those in severe-type kidneys. Scale bar, 1 mm. g Expressions of nephron segment markers in mild-type kidneys. The cyst areas that are positive for AQP1 or AQP2 in the cortex, the outer medulla, or inner medulla are surrounded by white lines. “L” indicates a left kidney and “R” indicates a right kidney. ND indicates not detected. Scale bar, 1 mm. h Box plot of the areas of AQP1-, AQP2-, NCC-, or UMOD-positive cysts in intermediate-type kidneys. “C” indicates the cortex, “OM” indicates the outer medulla, and “IM” indicates the inner medulla. n = 123 cysts in four animals. i Representative ECAD-positive and AQP1-, AQP2-, NCC-, and UMOD-negative cysts. Asterisks indicate the cysts. Scale bar, 100 µm. In the box plots, the top and bottom edges of boxes indicate the first and third quartiles, respectively; the center lines indicate the medians; and the ends of whiskers indicate the maximum and minimum values, respectively. Source data are provided as a Source Data file.