Fig. 2: APOBEC3A expression does not correlate with APOBEC activity monitored by DNA deaminase activity assay.
From: Quantification of ongoing APOBEC3A activity in tumor cells by monitoring RNA editing at hotspots
a Analysis of A3A and A3B mRNA expression in a panel of cancer cell lines determined by RT-qPCR. Error bar: S.D. (n = 3). b A3B levels in the indicated cancer cell lines were analyzed by western Blotting. c Schematic representation of DNA oligonucleotides used in this study. polyA-TC: oligonucleotide without any secondary structure (single-stranded DNA). NUP93: natural occurring DNA hairpin in the gene NUP93. d Deamination activity on PolyA-TC and NUP93 substrates from indicated cell extracts (30 μg). e A3A and/or A3B knockdown in BICR6 cells. Deamination activity in BICR6 cell extracts (30 μg) was monitor after indicated knockdown on both PolyA-TC and NUP93 substrates. Source data are provided as a Source Data file.
