Fig. 6: YBX1 correlates with, binds to and activates the promoters of RRM2, TK1, and TYMS.

a YBX1 mRNA expression significantly and positively correlates with RRM2 mRNA expression in n = 374 hepatocellular carcinoma patient samples (TCGA LIHC, indicated: log2 expression). Significance was calculated by unpaired, two-tailed t test. b YBX1 mRNA expression significantly and positively correlates with TK1 mRNA expression in n = 374 hepatocellular carcinoma patient samples (TCGA LIHC, indicated: log2 expression). Significance was calculated by unpaired, two-tailed t test. c YBX1 mRNA expression significantly and positively correlates with TYMS mRNA expression in n = 374 hepatocellular carcinoma patient samples (TCGA LIHC, indicated: log2 expression). Significance was calculated by unpaired, two-tailed t test. d The binding of YBX1 to the promoters of RRM2, TK1, and TYMS was determined by chromatin immunoprecipitation (ChIP) in HLE cells. Bar graph represents qPCR data after YBX1 ChIP compared with IgG as a negative control (n = 3). Data represent mean, and error bars represent SEM. Significance was calculated by unpaired, two-tailed t test where *P < 0.05; **P < 0.01; ***P < 0.001. e Luciferase assays show decreased transactivation of RRM2, TK1, and TYMS promoter regions after lincNMR or YBX1 silencing with 10 nM siPOOLs in HLE cells (n = 4). Data shown are ratios of firefly luciferase divided by Renilla luciferase used for standardization. Data represent mean, and error bars represent SEM. Significance was calculated by unpaired, two-tailed t test where *P < 0.05; **P < 0.01; ***P < 0.001.