Abstract
The ASCC3 subunit of the activating signal co-integrator complex is a dual-cassette Ski2-like nucleic acid helicase that provides single-stranded DNA for alkylation damage repair by the α-ketoglutarate-dependent dioxygenase AlkBH3. Other ASCC components integrate ASCC3/AlkBH3 into a complex DNA repair pathway. We mapped and structurally analyzed interacting ASCC2 and ASCC3 regions. The ASCC3 fragment comprises a central helical domain and terminal, extended arms that clasp the compact ASCC2 unit. ASCC2–ASCC3 interfaces are evolutionarily highly conserved and comprise a large number of residues affected by somatic cancer mutations. We quantified contributions of protein regions to the ASCC2–ASCC3 interaction, observing that changes found in cancers lead to reduced ASCC2–ASCC3 affinity. Functional dissection of ASCC3 revealed similar organization and regulation as in the spliceosomal RNA helicase Brr2. Our results delineate functional regions in an important DNA repair complex and suggest possible molecular disease principles.
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Introduction
The human genome is constantly under assault by endogenous or exogenous DNA damaging agents. To ward off these insults, cells have evolved systems to recognize DNA damage, signal its presence and initiate repair processes1. Among the diverse repair mechanisms, direct DNA repair processes represent efficient means to revert chemical changes to DNA and involve enzymes such as photolyases, alkyl-transferases or dioxygenases2,3. Escherichia coli α-ketoglutarate-dependent dioxygenase, AlkB, homologs (AlkBH’s) are one class of important DNA repair factors that reverse N-alkyl lesions4. Among the nine identified AlkBH enzymes in human, AlkBH2 and AlkBH3 de-alkylate 1-methyl adenosine and 3-methyl cytosine5,6.
Several lines of evidence implicate the human activating signal co-integrator complex (ASCC) in AlkBH3-mediated DNA repair. ASCC is composed of four subunits, ASCC1, ASCC2, ASCC3, and ASC1/TRIP4 (refs. 7,8). ASCC3 is the largest subunit of ASCC and was characterized as a DNA helicase that unwinds DNA by translocating on one strand in 3’-to-5’ direction8. The enzyme is thought to provide single-stranded DNA as a substrate for de-alkylation repair by AlkBH3 (ref. 8). ASCC and alkylated nucleotides co-localize at nuclear foci upon alkylation damage stress, dependent on a coupling of ubiquitin conjugation to ER degradation (CUE) domain in ASCC2, which links DNA alkylation damage repair to upstream ubiquitin signaling via the RING finger protein 113A9. ASCC1 is cleared from these foci upon DNA alkylation damage and knockout of ASCC1 leads to loss of ASCC2 from the nuclear foci and increased cellular sensitivity to alkylating insults10.
Both ASCC2 and ASCC3 have been linked to various human diseases. ASCC2 is upregulated in patients with rheumatoid arthritis11, and ASCC3 is upregulated in peripheral blood mononuclear cells from patients with lung cancer12,13. A role of ASCC3 in cancer development or progression is also suggested by the observation that knockdown of ASCC3 in a prostate cancer cell line suppresses cell proliferation8. Moreover, the Catalog Of Somatic Mutations In Cancer (COSMIC v91, release date 07. April 2020; https://cancer.sanger.ac.uk/cosmic)14 lists 223 and 652 somatic nonsense/missense/frame shift mutations in the ascc2 and ascc3 genes, respectively (822 and 2197 tested human cancer samples, respectively). As cancers can be caused by the acquisition of somatic mutations15, the large number of somatic ascc2 and ascc3 mutations observed in cancer samples might be linked to the roles of ASCC/AlkBH3 in sensing and repairing alkylated DNA lesions. Moreover, chemotherapy is one of the most widely used cancer treatments and causes various DNA lesions, including alkylation damage16. The functional interplay of ASCC and AlkBH3 in DNA de-alkylation repair may thus also have repercussions for cancer chemotherapy9.
Apart from DNA de-alkylation repair, ASCC has been implicated in a number of other cellular processes. The complex was originally discovered as a co-activator of nuclear hormone receptors17. In addition, ASCC3 has been implicated in viral defense mechanisms18,19,20,21. Finally, a genome-wide CRISPRi screen showed that ASCC2 and ASCC3 are the two most potent modifiers of cell fitness in the presence of a translation inhibitor, suggesting that the proteins affect stalled ribosomes22. Indeed, ASCC3 has been suggested to resolve stalled ribosomes on poly-A sequences in a ribosome quality control pathway23,24,25. Likewise, its yeast homolog, Slh1p, has been implicated in ribosome-associated quality control to prevent the accumulation of aberrant proteins23,26,27 and in non-functional ribosomal RNA decay28.
As a member of the Ski2-like family of superfamily (SF) 2 nucleic acid helicases, ASCC3 shows striking resemblance to the pre-mRNA splicing factor Brr2 (sequence identity of 41/33% between human ASCC3 and human/yeast Brr2), an ATP-dependent RNA helicase that is essential for spliceosome activation, catalysis and disassembly29. Based on the well-documented structural organization of Brr2 (refs. 30,31,32,33), ASCC3 is predicted to encompass a large N-terminal region (NTR; residues 1–400) and a helicase region (HR; residues 401–2202) containing a tandem array of two Ski2-like helicase cassettes. Each helicase cassette is predicted to encompass two RecA-like domains, a winged-helix domain and a Sec63 homology unit; the latter elements comprise sequential helical bundle or “ratchet”, helix-loop-helix and immunoglobulin-like domains. Brr2 is a subunit of the U4/U6•U5 tri-snRNP34,35. ATPase/helicase activities of Brr2 are tightly regulated both intrinsically and by interacting proteins33,36,37,38,39. The high sequence similarity between ASCC3 and Brr2, and the observation that ASCC3, like Brr2, interacts with a number of other proteins, suggest that ASCC3 might be regulated by similar principles.
Structural knowledge regarding ASCC subunits and their various interactions will be instrumental in further delineating the molecular mechanisms by which these proteins participate in diverse cellular functions and how they are linked to human diseases. While some interacting regions among ASCC subunits have been broadly mapped9,10, the structural basis of these interactions is presently unclear. Currently, only an unpublished NMR structure of the isolated ASCC2 CUE domain (PDB ID 2DI0) is known.
Here, we mapped precise interacting regions of the two largest ASCC subunits, ASCC2 and ASCC3, and determined a crystal structure of an ASCC2–ASCC3 complex comprising these portions. Guided by the structure, we delineated segments and residues that are critical for the ASCC2–ASCC3 interaction, which include a large number of residues that are altered by somatic cancer mutations. We also further investigated ATPase/helicase activities of ASCC3, and how they may be impacted by ASCC2.
Results
Experimental definition of a stable, minimal ASCC2–ASCC3 complex
Full-length ASCC2 (ASCC2FL) and two fragments of ASCC3, encompassing the NTR (ASCC3NTR; residues 1–400) and helicase region (ASCC3HR; residues 401-2202), were obtained by recombinant expression in insect cells. Analytical size exclusion chromatography (SEC) showed that ASCC2 interacts with ASCC3NTR (Fig. 1a) but not with ASCC3HR (Fig. 1b), consistent with previous reports9. The complex assembled from ASCC2FL and ASCC3NTR failed to crystallize. To remove putatively flexible regions that may hinder crystallization, we subjected the ASCC2FL–ASCC3NTR complex to limited proteolysis and mapped stable fragments by mass spectrometric fingerprinting. Elastase digestion gave rise to an approximately 50 kDa fragment of ASCC2, containing the first 434 residues (ASCC21–434), and an N-terminal, 207-residue fragment of ASCC3 (ASCC31–207), which maintained stable interaction in SEC (Fig. 1c). The ASCC21–434–ASCC31–207 complex was produced by recombinant co-expression in insect cells, purified and yielded diffracting crystals.
a, b SDS-PAGE analysis of SEC runs monitoring interaction of ASCC2FL and ASCC3NTR (a) and lack of interaction between ASCC2FL and ASCC3HR (b). In this and following figures: kDa, molecular weights of molecular weight markers in kDa. c SDS-PAGE analysis of elastase treatment of the ASCC2FL-ASCC3NTR complex. Bands running between ASCC2FL and ASCC21–434 represent intermediate ASCC2 fragments that occur transiently in the course of the digestion. Experiments shown in a–c have been repeated independently at least three times with similar results. d Orthogonal cartoon plots of ASCC21–434. ASCC2-N, N-terminal extension, slate blue; helices in sub-domain A, beige and violet; helices in sub-domain B, orange and purple. Helices are labeled as in the text. N, N-terminus; C, C-terminus. e Schemes of the domain organizations of ASCC21–434 and ASCC31–207. Violet and purple bars in the ASCC21–434 scheme represent helices h3a/b and h6a/b. The C-terminal 21 residues of ASCC31–207 (gray line) are not defined in the electron density. f Orthogonal views on the ASCC21–434-ASCC31–207 complex, with ASCC21–434 as surface view and ASCC31–207 as cartoon. ASCC21–434, colored as in d. N-arm of ASCC31–207, blue; C-arm of ASCC31–207 complex, red; helical domain of ASCC31–207, lime green.
Structure analysis and overall architecture
The crystal structure of the ASCC21–434-ASCC31–207 complex was determined via the single wavelength anomalous dispersion (SAD) approach, using a complex reconstituted by SEC from seleno-methionine (SeMet)-derivatized ASCC21–434 produced in E. coli and ASCC31–207 produced in insect cells (Supplementary Fig. 1). The structure was refined at 2.7 Å resolution to Rwork/Rfree values of 20.4/24.7% with good stereochemistry (Supplementary Table 1). An asymmetric unit of the crystals contains one ASCC21–434–ASCC31–207 complex. In the final model, we traced residues 2–408 of ASCC21–434 with a gap between residues 216–226 representing a flexible loop. The ASCC31–207 model comprises residues 1–186 without gaps and two additional residues at the N-terminus that were retained after tag cleavage.
Within the complex, the structure of ASCC21–434 exhibits an irregularly structured N-terminal region (residues 2–50), and two similarly structured, helical sub-domains (sub-domain A, residues 51–214; sub-domain B, residues 241–406; Fig. 1d, e). Both sub-domains are composed of eight α-helices (h1a-h8a/h1b-h8b; Fig. 1d). Within each sub-domain, h1/h2, h4/h5, and h7/h8 form helical hairpins (beige/orange in Fig. 1d) that are connected by h3 and h6 (pink/magenta in Fig. 1d). h3a is significantly shorter than h3b (13 vs. 26 residues, respectively) and connected to h4a by a 13-residue, irregularly structured loop, as opposed to the single-residue connection between h3b and h4b. Furthermore, helices h7a and h8a are pried open compared to the h7b-h8b equivalents, due to helix h1b that wedges in between them (Fig. 1d).
The ASCC31–207 fragment folds into a central helical domain (residues 46–159) and extended arms at the N-termini and C-termini (N-arm, residues 1–45, C-arm, residues 160–186), with which it clasps the compact ASCC21–434 (Fig. 1e, f). The N-arm of ASCC31–207 forms three helices, h1, h2 and h3, preceded and connected by irregularly structured linkers along one flank of ASCC21–434 (Fig. 1f). The first helix (h1, residues 5–13) is embedded between helices h6a and h8a of ASCC21–434 sub-domain A and helix h1b of ASCC21–434 sub-domain B. Helix h2 (residues 15–21) runs across ASCC21–434 helix h3a. Helix h3 (residues 26–40) secures part of the N-terminal extension of ASCC21–434 between helices h1a and h3a of ASCC21–434 sub-domain A (Fig. 1f). The central helical region of ASCC31–207 consists of six helices (h4-h9). Helices h4, h5, h7, h8, and h9 are arranged in a circle around the central helix h6, in front of helix h2a of ASCC21–434 (Fig. 1f). The C-arm stretches in an extended, irregular conformation along the opposite flank of ASCC21–434, connecting sub-domains A and B of ASCC21–434 (Fig. 1f).
Interfaces between ASCC3 and ASCC2 are evolutionarily conserved
Structurally or functionally important regions in proteins are often evolutionarily conserved. We analyzed conservation of the ASCC21–434–ASCC31–207 contact regions using the ConSurf server40. This analysis revealed that residues located at ASCC21–434–ASCC31–207 interface regions are highly conserved (Fig. 2). Residues 91, 93, 103, 163, and 251 of ASCC21–434 were assigned the highest conservation scores. These residues are located in helix h3a, h6a and h1b, and form a binding pocket for helix h1 of the ASCC31–207 N-arm (Fig. 2a, left). ASCC21–434 residues that form a binding surface for the ASCC31–207 C-arm are conserved to a lesser extent (Fig. 2a, right). Mirroring the conservation pattern on ASCC21–434, residues of the ASCC31–207 N-arm, in particular the first 16 residues, are significantly more conserved compared to other surface positions of the protein (Fig. 2b). This analysis suggests that interactions involving the N-arm of ASCC31–207 may be of particular importance for the formation of the ASCC21–434-ASCC31–207 complex, and that the ASCC21–434–ASCC31–207 interaction observed here is likely conserved in all organisms that contain these proteins.
a Residue conservation mapped to the surface of ASCC21–434 with ASCC31–207 shown as cartoon (colored as in Fig. 1f). b Residue conservation mapped on the structure of ASCC31–207 shown as cartoon, with ASCC21–434 in surface view (beige). Central legend, color code representing the degree of conservation of individual residues. Views as in Fig. 1d, f.
The N-terminal arm of ASCC3 is essential for stable binding to ASCC2
To elucidate the relative importance of different regions of ASCC31–207 for interaction with ASCC21–434, we used structure-informed mutagenesis in combination with analytical SEC and isothermal titration calorimetry (ITC). First, we designed sequential N-terminal truncations of ASCC31–207 with or without the C-arm. ASCC3 fragments lacking C-terminal residues not visible in the structure (ASCC31–197) or additionally lacking the C-arm (ASCC31–161) maintained stable interaction with ASCC21–434 in SEC (Fig. 3a). By contrast, deletion of the N-arm (ASCC342–197) abolished stable binding to ASCC21–434 in SEC (Fig. 3b), while a partial N-arm deletion variant (ASCC316–197) still co-migrated with ASCC21–434 in SEC (Fig. 3c).
a–c SDS-PAGE analysis monitoring SEC runs of the indicated mixtures of proteins. d–h ITC runs monitoring the interaction between the indicated pairs of proteins. Deduced Kd values are listed as means ± SD for runs for which affinities could be quantified. i Western blot monitoring pulldown of ASCC2 by the indicated ASCC3 constructs from cell extracts. j Quantification of the data shown in i. Columns represent means relative to ASCC3FL of n = 2 independent experiments, using the same biochemical samples. Open circles, individual measurements.
Quantifying binding affinities by ITC revealed a similar, yet more detailed, picture. ITC showed a Kd of 3.5 nM for the interaction of the complete ASCC3NTR and ASCC2FL (Fig. 3d). ASCC31–197 (lacking the C-terminal 10 residues of ASCC31–207 but containing all ASCC3 residues with well-defined electron density in the ASCC21–434-ASCC31–207 complex structure) and ASCC21–434 interacted with a similar affinity (Kd of 3.8 nM), suggesting that the fragments contained in our crystal structure encompass the entire ASCC2–ASCC3 interacting regions (Fig. 3e). Deletion of the entire C-arm of ASCC31–207 (ASCC31–161) led to an approximately 14-fold decreased affinity (Kd = 47.7 nM; Fig. 3f). Truncation of the N-terminal 15 residues that form helix h1 in the N-arm of ASCC31–197 reduced affinity to ASCC21–434 by more than two orders of magnitude (Kd = 483.0 nM; Fig. 3g). Lack of the entire N-arm of ASCC31–197 (ASCC342–197) completely abrogated the interaction, as no signal was detected in ITC measurements (Fig. 3h). Together, the above results indicate that the N-arm, and in particular the first 15 residues, of ASCC31–207 are essential for a stable binary interaction with ASCC21–434, consistent with its high degree of evolutionary conservation. The C-arm of ASCC31–207 contributes to the interaction with ASCC21–434 but it is not essential for the proteins to maintain a stable complex in SEC, consistent with a reduced but still high level of evolutionary conservation of the C-arm.
We next tested whether the interaction pattern observed using recombinant proteins in vitro also applies to the ASCC2–ASCC3 interaction in living cells. Expression constructs encoding C-terminally flag-tagged ASCC3FL and stepwise N-terminally truncated fragments (ASCC316-end, ASCC342-end, ASCC3207-end, and ASCC3401-end) were used to generate stably transfected HEK293 Flp-In cell lines for the tetracycline-inducible expression of these proteins. After induction, flag-tagged ASCC3 or fragments were captured on anti-flag beads and co-precipitation of ASCC2 was monitored by western blotting. In line with our in vitro interaction mapping, ASCC3 variants with increasing N-terminal deletions co-precipitated stepwise reduced amounts of ASCC2 (Fig. 3i). ASCC3 variants lacking 15, 41, 206, or 400 N-terminal residues, co-precipitated 73, 40, 26, or 16%, respectively, of the amount of ASCC2 associated with full-length ASCC3 (Fig. 3j). While based on our structural and mutagenesis data truncation of the N-terminal 206 or 400 residues of ASCC3 should abrogate direct ASCC2–ASCC3 interactions, ASCC3207-end and ASCC3401-end most likely still pulled down reduced amounts of ASCC2 (Fig. 3i, j), because other ASCC subunits also mediate indirect ASCC2–ASCC3 interactions in vivo.
ASCC2 and ASCC3 residue substitutions found in human cancers cluster at interfaces and lead to reduced affinities
223 and 652 somatic nonsense, missense or frame shift mutations in human cancer cell or tissue samples have been mapped to the ascc2 and ascc3 coding regions, respectively (https://cancer.sanger.ac.uk/cosmic). 123 and 95 of these mutations affect residues in ASCC21–434 and ASCC31–207, respectively. Strikingly, 16 of the missense mutations and three nonsense mutations affect residues E53, R58, T60, D65, L70, R96, Y97, D103, or R121 in ASCC21–434, and 17 or one, respectively, of these mutations affect residues R5, R11, S12, D28, R33, K165, or E181 in ASCC31–207, which all constitute direct contact points between the proteins in our crystal structure (Fig. 4a). Many of the remaining point mutations map to, and are expected to disturb, the globular portions of the proteins. In addition, there are three frameshift mutations that would affect almost the entire ASCC21–434 region or large parts thereof (E30, F124, G171 in ASCC2; Fig. 4a). 25 frameshift mutations map to residues F163 and G164 in ASCC3, affecting the entire C-arm of ASCC31–207 (Fig. 4a).
a Orthogonal views on the ASCC21–434-ASCC31–207 structure, highlighting interface residues and frameshift positions affected by somatic cancer mutations. ASCC21–434, beige; ASCC31–207, lime green; ASCC2 residues, brown sticks; ASCC3 residues, dark green sticks; first positions affected by frameshift mutations, red. Orientations of the panels as in Fig. 1d,f. b Details of the interaction networks involving cancer-related residues D103 of ASCC2, as well as R5 and R11 of ASCC3. Dashed lines, salt bridges. Rotation symbol, view relative to Fig. 1d,f, left. c–i ITC runs monitoring the interaction of ASCC21–434 with the indicated ASCC31–22 peptide variants. Deduced Kd values are listed as means ± SD for runs for which affinities could be quantified.
We thus surmised that many residue substitutions of ASCC21–434 or ASCC31–207 found in human cancers affect the ASCC2–ASCC3 affinity. To test this hypothesis, we conducted comparative ITC analyses using ASCC21–434 and peptides representing the N-terminal 22 residues of ASCC3 exhibiting wild-type (WT) sequence or residue substitutions R5G, R5C, R5H, R5L, R11C, or R11H. R5 residue substitutions are found in large intestine and lung adenocarcinomas, as well as in cervical and esophageal squamous cell carcinomas, while the R11 residue substitutions occur in endometrioid carcinoma and large intestine adenocarcinoma (https://cancer.sanger.ac.uk/cosmic). In our crystal structure, R5 of ASCC3 is positioned at the N-terminus of helix h1 and interacts with D103 of ASCC21–434 (D103 of ASCC21–434 is also affected by somatic cancer mutations), while R11 of ASCC3 lies at the C-terminal end of helix h1 and engages in hydrogen bonds and/or salt bridges with D63 and D92 in ASCC21–434 (Fig. 4b). We used ASCC3 peptides in these experiments as the high affinity observed in ITC between ASCC21–434 and ASCC31–197 or longer ASCC3 variants (Fig. 3d, e) may mask differences due to single residue substitutions.
WT ASCC31–22 bound to ASCC21–434 with a Kd of 2.0 µM (Fig. 4c). ASCC31–22 peptides bearing R5L or R5G substitutions weakened the interaction with ASCC21–434 approximately eight-fold and eleven-fold, respectively (Fig. 4d, e). ASCC31–22 peptides comprising R5H or R5C substitutions showed more than 20-fold reduced affinities compared to WT (Fig. 4f, g). R11H or R11C substitutions in ASCC31–22 completely eradicated binding to ASCC21–434 (Fig. 4h, i). These observations are consistent with the notion that reduced affinity to ASCC2 represents a means by which of R5 and R11 residue substitutions in ASCC3 contribute to cancer phenotypes.
The N-terminal cassette is an active helicase unit in ASCC3
ASCC3 bears close resemblance to the spliceosomal RNA helicase, Brr2. Both ASCC3 and Brr2 contain an approximately 400-residue NTR followed by two Ski2-like helicase cassettes with identical domain composition (Fig. 5a, left). It is well documented that the N-terminal Ski2-like helicase cassette constitutes the active helicase unit in Brr2, while the C-terminal cassette lacks ATPase and helicase activities32. The reverse situation has been reported for human ASCC3; in contrast to an inactive isolated N-terminal cassette construct, an isolated C-terminal cassette construct was found to be active in DNA duplex unwinding8. As we did not perceive alterations in conserved helicase motifs in the N-terminal cassette that would obviously preclude helicase activity (Fig. 6a), we revisited the question of helicase activity of the ASCC3 helicase cassettes.
a Comparison of the structure of full-length yeast Brr2 (left) and the present ASCC21–434-ASCC31–207 complex (right) after superposition of the plug/plug-like domains (blue). Human Brr2 in the U4/U6•U5 tri-snRNP or in the pre-catalytic spliceosome exhibits a plug domain very similar to yeast Brr2 blocking RNA access42,43 (PDB IDs 3JCR, 6QW6, 6QX9). NC, N-terminal cassette; CC, C-terminal helicase cassette. b Experimental setup for stopped-flow/fluorescence-based unwinding assays. Gray sphere, helicase; star symbol, fluorophore (Alexa 488); Q, quencher (Atto 540 Q). c Stopped-flow/fluorescence-based assays monitoring unwinding of a 3’-overhang RNA by Brr2FL or Brr2HR, showing that auto-inhibition, which has been documented for Brr2 using gel-based unwinding assays33, can be readily detected using the present experimental setup. d Stopped-flow/fluorescence-based assays monitoring unwinding of DNA bearing a 3’-overhang (3’-ovh), and lack of unwinding of a 5’-overhang (5’-ovh) DNA by ASCC3HR upon addition of ATP. e Nucleotide preference of ASCC3HR in unwinding a 3’-overhang DNA. f Stopped-flow/fluorescence-based assays monitoring unwinding of 3’-overhang DNA by ASCC3NC compared to ASCC3HR using ATP. g Stopped-flow/fluorescence-based assays monitoring unwinding of a 3’-overhang DNA by the indicated ASCC3 constructs in the absence or presence of ASCC2 using ATP. Insets, side-by-side presentation of the largely overlapping curves. h Stopped-flow/fluorescence-based assays monitoring unwinding of a 3’-overhang DNA by ASCC3HR alone or in the presence of ASCC3NTR or ASCC342–161 using ATP. Insets, side-by-side presentation of the largely overlapping curves. FL/HR/NC/NTR/(42–161), ASCC3 variants as defined in the text.
a Multiple sequence alignment of conserved helicase motifs (indicated by letters or Roman numerals above the alignment) in human ASCC3, human Brr2 and yeast Slh1p N-terminal cassettes (NC) and C-terminal cassettes (CC). The conserved motif I lysine and motif II aspartate residues of ASCC3 cassettes, which were altered in the present analysis, are highlighted in blue and red, respectively. Color coding of the motifs: involved in ATP binding, blue; involved in RNA binding, yellow; involved in communication of RNA binding and ATPase activities, red. Roles of selected residues are indicated below the alignment. b–d Stopped-flow/fluorescence-based assays monitoring unwinding of 3’-overhang DNA by the indicated ASCC3HR variants using ATP. Schemes below the unwinding traces represent ASCC3HR with altered residues highlighted. WT/K505N/D611A/K1355N/D1453A/KK505/1355NN/DD611/1453AA, ASCC3HR variants as defined in the text.
While we were able to produce a recombinant ASCC3 fragment encompassing the N-terminal cassette (ASCC3NC; residues 401–1300), a fragment containing only the C-terminal unit could not be produced in soluble form. To test helicase activities, we pre-incubated ASCC3 variants (see below) with a 12-base pair DNA duplex containing a 31-nucleotide 3’-single-stranded overhang, and bearing a fluorophore (Alexa Fluor 488) on the 3’-terminus of the short strand and a quencher (Atto 540 Q) on the 5’-terminus of the long strand (Fig. 5b). As a control, we first tested the assay with Brr2 variants and an analogous RNA duplex. Fast mixing of Brr2FL or Brr2HR (residues 395–2129; a truncated version of Brr2 lacking most of the auto-inhibitory NTR, largely equivalent to ASCC3HR) pre-incubated with the RNA substrate and ATP in a stopped-flow device led to time-dependent increases in the Alexa Fluor 488 fluorescence, indicating RNA duplex unwinding (Fig. 5c). Unwinding occurred at a significantly higher rate with Brr2HR than with Brr2FL (Fig. 5c), showing that the stopped-flow/fluorescence setup can reliably monitor differences in helicase activity between alternative dual-cassette helicase constructs. As expected, ASCC3HR efficiently unwound a DNA duplex with 3’-single stranded overhang, but not a duplex with 5’-single stranded overhang (Fig. 5d), and preferentially used ATP for DNA unwinding (Fig. 5e). Surprisingly, however, in our hands, ASCC3NC also exhibited helicase activity, albeit reduced compared to ASCC3HR (Fig. 5f). These findings were confirmed using gel-based unwinding assays (Supplementary Fig. 2).
Nucleic acid helicases contain a number of conserved, functionally important sequence motifs in their RecA domain cores (Fig. 6a). In particular, a lysine in motif I is required for ATP binding, while an aspartate in motif II is crucial for coordinating a magnesium ion to trigger the hydrolysis of ATP (Fig. 6a), and substitutions of these residues are expected to strongly interfere with helicase activity. To further test the contributions of the N-terminal and C-terminal cassettes to the helicase activity of a dual-cassette ASCC3 construct, we individually substituted the corresponding lysine and aspartate residues in the N-terminal cassette (K505, D611), in the C-terminal cassette (K1355, D1453) or in both cassettes of ASCC3HR with alanine or asparagine, respectively. We verified the intended mutations by sequencing the final generation of baculoviruses used for production of the ASCC3HR variants (Supplementary Fig. 3). ASCC3HR variants that contained mutations in motifs I or II of the N-terminal cassette had strongly reduced or no detectable helicase activities (Fig. 6b). By contrast, ASCC3HR variants that contained residue substitutions K1355N or D1453A within the C-terminal cassette retained the activity of WT ASCC3HR (Fig. 6c). Again, gel-based unwinding assays using D611A and D1453A variants of ASCC3HR were consistent with these results (Supplementary Fig. 2). Also consistent with these findings, DNA helicase activities of the K505N/K1355N and D611A/D1453A double variants were almost fully abrogated (Fig. 6d). These results suggest that, contrary to previous observations8 and similar to Brr2 (ref. 32), the N-terminal cassette of ASCC3 is an active helicase in vitro. As the ASCC3NC helicase activity was reduced compared to that of ASCC3HR (Fig. 5f; Supplementary Fig. 2) it remains to be seen whether the C-terminal cassette is inactive but stimulates helicase activity of ASCC3NC, as has been observed for Brr2 (ref. 32), or whether ASCC3CC is an active helicase as well that contributes to the overall activity of ASCC3HR.
ASCC2 does not influence the helicase activity of ASCC3
The Brr2 NTR contains helical “plug” and PWI-like domains connected by extended, intrinsically disordered regions33,41. In isolated yeast Brr2 (ref. 33), as well as in human Brr2 in the U4/U6•U5 tri-snRNP or in the pre-catalytic spliceosome42,43, the NTR or part of it can fold back onto the helicase region, with the plug domain blocking substrate RNA loading (Fig. 5a, left), thereby auto-inhibiting Brr2 ATPase and helicase activities. Having established a similar functional organization of the helicase regions of ASCC3 and Brr2, we sought to investigate whether ASCC3 is also auto-regulated via its NTR.
While the overall sequence identity between human ASCC3 and human Brr2 is around 41%, the sequence identity is lower for their NTRs, around 20%. Despite the lower level of sequence conservation, structural comparison of ASCC31–207 with the NTR of yeast and human Brr2 revealed that a central, four-helix portion of ASCC31–207 (residue 59–142) closely resembles the Brr2 plug domain (root-mean-square deviation [rmsd] of 2.7 Å for 36 pairs of common Cα atoms; Fig. 5a). However, in our crystal structure, the ASCC31–207 arms neighboring the plug-like domain extend along ASCC21–434 (Fig. 5a, left); thus, when bound to ASCC2, these regions would not be available to fold back on the ASCC3 helicase region.
The helicase activity of ASCC3FL was significantly reduced compared to ASCC3HR, consistent with similar auto-inhibition as for Brr2 (Fig. 5g). When added in trans, ASCC3NTR or the plug domain-containing fragment, ASCC342–161, did not alter ASCC3HR helicase activity (Fig. 5h), indicating that the NTR has to be covalently connected to the helicase region to elicit the auto-inhibitory effect. Addition of ASCC2 did not influence the helicase activity of ASCC3FL (Fig. 5g). Due to the high affinities quantified for the ASCC2FL-ASCC3NTR and ASCC21–434-ASCC31–197 interactions (Fig. 3d, e), we consider it unlikely that the NTR is sequestered from ASCC2 by interacting in a mutually exclusive manner with the helicase region. Instead, the plug-like domain in ASCC3 may still be able to occupy an inhibitory position when associated with ASCC2.
Discussion
In this study, we have delineated interacting regions of the ASCC2 and ASCC3 subunits of the ASCC DNA repair machinery, and elucidated the structural basis for this interaction. Guided by our structural results, we identified regions and residues of the two proteins that significantly contribute to stable complex formation. The importance of regions for the interaction scales with the degree of their evolutionary conservation. Moreover, we observed that a large number of ASCC2 and ASCC3 residue substitutions encoded by somatic mutations in cancers map to ASCC2–ASCC3 interface regions and that selected, cancer-related residue substitutions in ASCC3 lead to reduced ASCC2–ASCC3 affinity. We also conducted in vitro enzymatic analyses to better characterize the ASCC3 nucleic acid helicase, showing (i) that the N-terminal cassette represents an active helicase, (ii) that helicase activity in the C-terminal cassette is dispensable for the activity of the full-length protein, (iii) that helicase activity in ASCC3 is auto-inhibited by the NTR, and (iv) that interaction with ASCC2 does not strongly influence ASCC3 DNA unwinding activity in vitro.
ASCC3 and Brr2 both belong to a sub-group of the Ski2-like family of SF2 helicases with a tandem array of complex helicase units. The helicase regions of ASCC3 and Brr2 seem to be organized in an analogous fashion, comprising an active N-terminal and an inactive, or at least largely dispensable, C-terminal helicase cassette. Consistent with this notion, an ASCC3 variant bearing an ATPase-disrupting substitution in the C-terminal helicase cassette, but not in the N-terminal cassette, can rescue a ribosome poly-A readthrough phenotype elicited by ASCC3 knockdown25. Likewise, in the yeast ASCC3 homolog, Slh1p, residue substitutions in the N-terminal cassette can abrogate ribosome quality control function in vivo27.
Several proteins have been identified that directly bind to the helicase region of Brr2 and modulate its helicase activity, including a C-terminal Jab1-like domain of the Prp8 protein36 and a largely intrinsically unstructured protein, FBP21 (ref. 38). ASCC1 interacts with the helicase region of ASCC3 but not with the NTR10. It will be interesting to see in future studies, which of the cassettes it interacts with, whether it modulates the ASCC3 helicase upon binding and whether similar molecular principles as in the case of Brr2 and its protein co-factors apply to such putative regulation of ASCC3.
Our structural analysis revealed that ASCC3 and Brr2 share at least some structural organization in their NTRs as well. Both contain a plug/plug-like domain close to their N-termini (residues 59–142 in human ASCC3, 113–192 in yeast Brr2, 107–180 in human Brr2). In Brr2, the plug domain contributes to auto-inhibition of the isolated enzyme33 and our results suggest similar auto-inhibition in isolated ASCC3. Based on our crystal structure, regions neighboring the ASCC3 plug-like domain are expected to be guided away from the ASCC3 helicase region when bound to ASCC2, however, ASCC2 did not alleviate ASCC3 auto-inhibition. ASCC3 auto-inhibition may, therefore, be predominantly mediated via the plug-like domain, which remains accessible in complex with ASCC2, occupying a position on the helicase region where it competes with DNA substrate loading. Still, our data suggest that a covalent connection of the plug-like domain to the helicase region is required for auto-inhibition. Thus, the general principle of auto-inhibition via the NTR seems to be conserved between ASCC3 and Brr2, but details of the mechanisms may differ.
Correlation of the evolutionary conservation of ASCC2–ASCC3 interfaces and the contributions of these regions to a stable interaction underscore the functional importance of the complex we structurally analyzed. As the two proteins seem to participate together in various ASCC functions (transcription co-activation, DNA repair, ribosome-dependent protein quality control, viral defense), we expect that all of these functions depend on the observed ASCC2–ASCC3 interaction.
We observed an extraordinary number of residues affected by somatic cancer mutations at ASCC2–ASCC3 interfaces, and found that some cancer-related residue substitutions lead to reduced ASCC2–ASCC3 affinity. Based on these observations and previous insights into the role of ASCC components in DNA alkylation damage repair, we suggest that reduced ASCC2–ASCC3 affinity might contribute to malignant transformation. As DNA alkylation damage can occur non-enzymatically44,45, the increased metabolic activity of cancer cells may lead to a higher level of metabolic DNA damage46, rendering cancer cell proliferation dependent on systems that can repair excessive DNA damage. Indeed, ASCC3 is overexpressed in several cancers and ASCC3 knockdown, which would be expected to lead to less efficient AlkBH3-mediated DNA de-alkylation repair, has been shown to negatively impact tumor cell proliferation in culture and xenograft models8,13. Other components of ASCC seem to contribute to efficient ASCC3/AlkBH3-mediated DNA alkylation damage repair by establishing a complex DNA damage signaling and repair pathway9,10. Specifically, ASCC1 appears to promote co-localization of ASCC2 and ASCC3 at nuclear foci during alkylation damage, and loss of ASCC1 leads to increased alkylation sensitivity10. Proper co-localization of ASCC2 and ASCC3, therefore, appears to be essential for efficient DNA alkylation damage repair. Similar to ASCC1 knockdown, reduced ASCC2–ASCC3 affinity, as elicited by several somatic cancer mutations, may influence ASCC2–ASCC3 co-localization at nuclear foci, leading to increased DNA alkylation damage. This model does not invoke a direct influence of ASCC2 on ASCC3 helicase activity, consistent with our findings. While reduced DNA alkylation damage repair due to reduced ASCC2–ASCC3 affinity would be expected to negatively impact cancer cell proliferation, DNA alkylation can represent mutagenic lesions44,45. Thus, reduced ASCC2–ASCC3 interaction may contribute to the initial development of a cancer cell phenotype, as increased DNA alkylation damage may lead to transforming mutations.
Methods
Molecular cloning
DNA regions encoding the proteins/protein regions of interest were PCR-amplified from synthetic genes (GeneArt), optimized for expression in Trichoplusia ni cells. Supplementary Tables 2 and 3 list sequences of PCR primers and synthetic genes, respectively. DNA regions encoding ASCC3FL, ASCC3NTR, ASCC3HR, ASCC3NC, ASCC31–207, or full-length ASCC2 were cloned into a modified pFL vector under control of the very late polyhedrin promoter, which directed the synthesis of fusion proteins with TEV-cleavable N-terminal His10-tags. A DNA fragment encoding ASCC21–434 was cloned into the pUCDM vector and Cre-recombined with ASCC31–207-encoding pFL. The QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) was used to introduce mutations that give rise to ASCC3HR variants K505N, K1355N, D611A, D1453A, K505N-K1355N, and D611A-D1453A. The expression cassettes were integrated into the MultiBac baculoviral genome via Tn7 transposition within a lacZα gene, allowing selection of recombinants by blue/white screening. Recombinant bacterial artificial chromosomes (BACs) were isolated from the bacterial hosts.
DNA fragments encoding ASCC3NTR, ASCC31–207, ASCC31–197, ASCC31–161, ASCC316–197, ASCC342–197, and ASCC21–434 were PCR-amplified from the pFL vectors encoding ASCC3NTR or full-length ASCC2. These fragments were inserted into the pETM-11 vector (EMBL, Heidelberg) that guides production of proteins with TEV-cleavable N-terminal His6-tags. All constructs were confirmed by sequencing.
Protein production and purification
ASCC21–434 was co-produced with ASCC31–207 in High Five cells. For virus production, the isolated BAC DNA was transfected into Sf9 cells in a six-well plate. After 68 h, the supernatant containing the initial virus (V0) was collected and used to infect a 50 ml suspension culture of Sf9 cells to amplify the virus (V1). For large scale production, 400 ml of High Five cell culture in a 2 l shaker flask was infected with 1 ml of V1 virus, and cells were harvested after 3–4 days. Production of the intended ASCC3HR variants was confirmed by sequencing of PCR-amplified regions from the V1 baculoviruses.
For production of isolated ASCC21–434 and of isolated N-terminal ASCC3 fragments, E. coli BL21 (DE3) cells were transformed with the corresponding pETM-11 vectors, cultivated in auto-inducing medium47 at 18 °C and harvested at an OD600 of ~10. SeMet-labeled ASCC21–434 protein was produced by auto-induction in cells grown in PASM-5052 medium48, which contained 10 µg/mg of unlabeled methionine and 125 µg/ml of SeMet. Cells were harvested by centrifugation and stored at −20 °C.
The same purification protocol was used for all individual proteins of interest (POIs) and for the ASCC21–434-ASCC31–207 used for crystallization, unless otherwise specified. Cell pellets were re-suspended in lysis buffer (20 mM HEPES-NaOH, pH 7.5, 500 mM NaCl, 10 mM imidazole, 1 mM DTT, 8.6% [v/v] glycerol), supplemented with protease inhibitors and lysed by sonication using a Sonopuls Ultrasonic Homogenizer HD (Bandelin). After centrifugation and filtration, the soluble fraction was incubated with Ni2+-NTA beads for 1 h at 4 °C, and loaded into a gravity flow column. After extensive washing of the beads with lysis buffer, the POI was eluted using lysis buffer containing 400 mM imidazole. The His6/10-tags of the POI was cleaved off during overnight dialysis into lysis buffer without imidazole at 4 °C. The protease-treated sample was reloaded on a fresh Ni2+-NTA gravity flow column to remove the His6/10-tag, uncleaved protein and the His-tagged protease. For crystallization, the flow-through was further purified by SEC on a Superdex 200 10/600 GL column (GE Healthcare) in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, 1 mM DTT. For other studies, the flow-through was further purified by SEC on a Superdex 200 10/600 GL column in 20 mM HEPES-NaOH, pH 7.5, 250 mM NaCl, 1 mM DTT, 5% (v/v) glycerol. For ASCC3NTR, the SEC step was omitted.
SeMet-labeled ASCC21–434–ASCC31–207 complex was assembled from individually purified, SeMet-labeled ASCC21–434 and unlabeled ASCC31–207 by incubation on ice for 30 min, followed by SEC on a Superdex 200 10/600 GL size-exclusion column (GE Healthcare). ASCC2FL–ASCC3NTR complex was formed and purified in the same way.
Limited proteolysis
5.7 µg of purified ASCC2FL-ASCC3NTR complex were incubated with increasing amounts (0.0013, 0.013, and 0.13 µg) of different proteases in 20 mM HEPES-NaOH, pH 7.5, 250 mM NaCl, 1 mM DTT, 5% (v/v) glycerol at 4 °C overnight. The reactions were stopped by adding SDS-PAGE loading buffer, and samples were separated by SDS-PAGE. Bands of interest were analyzed by in-gel trypsin digestion and mass spectrometric fingerprinting.
Crystallographic procedures
Unmodified or SeMet-modified ASCC21–434-ASCC31–207 complex was crystallized by the hanging drop vapor diffusion technique (0.5 µl protein at 5.8 mg/ml plus 1 µl reservoir) at 4 °C, using a reservoir that contained 0.1 M MES-NaOH, pH 6.5, 15% (w/v) polyethylene glycol 3350, 7% (v/v) 2-methyl-2,4-pentanediol, 3% (v/v) methanol. Crystals were cryo-protected by transfer into mother liquor containing 20% (v/v) propylene glycol, and flash-cooled in liquid nitrogen. Diffraction data were collected at beamline 14.1 of the BESSY II storage ring (Helmholtz-Zentrum Berlin, Germany) at 100 K. Diffraction data were processed with XDS49. The structure of ASCC21–434-ASCC31–207 complex was solved by the SeMet SAD strategy using phenix.autosol50. An initial model was calculated using phenix.autobuild51. The model was completed through alternating rounds of manual model building using Coot52 and automated refinement using phenix.refine53. Data collection, structure solution and refinement statistics are listed in Supplementary Table 1. Structure figures were prepared using PyMOL (Version 1.8 Schrödinger, LLC).
Analytical size exclusion chromatography
Proteins were produced and purified individually. 1 µmol ASCC21–434 was mixed with 2 µmol of ASCC31–161, ASCC342–197, or ASCC316–197 in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, 1 mM DTT to final volume of 50 µl. The mixtures were incubated on ice for 30 min and then chromatographed on a Superdex 200 3.2/300 analytical size exclusion column (GE Healthcare). Fifty microliter fractions were collected and subjected to SDS-PAGE analysis.
Isothermal titration calorimetry
ITC analyses were conducted on an iTC200 instrument (MicroCal). Proteins were dialyzed against ITC buffer (20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl). 300 µl of ASCC3 variant solution (10 µM according to UV absorption) were loaded into the sample cell, and were titrated with ASCC2 variant solution (100 µM) in the syringe via 18 injections of 1.5 µl each. Peptides representing the N-terminal 22 residues of ASCC3 (WT and R5H, R5C, R5G, R5L, R11H, R11C variants) were chemically synthesized (GL biochem) with C-terminal amination. Peptides were dissolved in ITC buffer. 300 µl peptide solution (10–15 µM according to weighing) were loaded into the sample cell, and were titrated with ASCC21–434 solution (200 µM or 400 µM) in the syringe via 16 injections of 2.5 µl each. All measurements were conducted at 25 °C. Data were analyzed with MicroCal PEAQ-ITC Analysis Software. For data analysis, stoichiometries were fixed to 1, as known from our crystal structure analysis, and the concentrations of ASCC3 variants or peptides were allowed to vary.
Generation of HEK293 Flp-In™ T-REx™ cell lines and immunoprecipitation of complexes
pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions. Cells in which the transgene had been genomically integrated into a specific locus were selected using hygromycin and blasticidin, and population cell lines were generated. Cells were grown in Dulbecco’s modified Eagle Medium supplemented with 10% fetal bovine serum at 37 °C with 5% CO2, and expression of the tagged proteins was induced by addition of 1 µg/ml tetracycline for 24 h before harvesting.
For immunoprecipitation of complexes containing Flag-tagged proteins54,55,56, cells expressing full-length or truncated ASCC3-Flag or the Flag tag alone were lysed by sonication in 50 mM TRIS-HCl, pH 7.4, 150 mM NaCl, 0.5 mM EDTA, 0.1% (v/v) Triton-X-100, 10% (v/v) glycerol and cOmpleteTM protease inhibitors (Roche). Cell debris were pelleted by centrifugation and the cleared lysate was incubated with anti-FlagM2 magnetic beads (Sigma-Aldrich) for 2 h. After thorough washing steps, complexes were eluted using 3xFlag peptide. Proteins were precipitated using 20% (w/v) trichloroacetic acid before separation by SDS-PAGE. Western blotting was performed using antibodies against ASCC2 (Proteintech, 11529-1-AP; diluted 1:1000) and the Flag tag (Sigma-Aldrich, F3165; diluted 1:7500). ASCC2 and ASCC3 bands were quantified using Image J software. The amount of ASCC2 relative to each ASCC3 fragment was calculated and normalized to the relative amount of ASCC2 co-precipitated with ASCC3FL (set at 1).
Stopped-flow/fluorescence-based helicase assays
DNA or RNA unwinding was monitored by fluorescence stopped-flow on a SX-20MV spectrometer (Applied photophysics). The DNA and RNA substrates contained a 12-base pair duplex region and a 31-nucleotide 3’-overhang (for sequences see Supplementary Table 4). They harbored an Alexa Fluor 488 moiety on the 3’-end of the short strand and an Atto 540 Q quencher on the 5’-end of the complementary strand, which were in close proximity after annealing. For negative control, we used a 5’-overhang DNA duplex with reverse sequences and labels (Supplementary Table 4). Experiments were conducted at 30 °C in 40 mM TRIS-HCl, pH 7.5, 80 mM NaCl, 0.5 mM MgCl2. 250 nM protein were pre-incubated with 50 nM DNA or RNA duplex for 5 min at 30 °C. 60 µl of the protein–DNA or protein–RNA mixture were rapidly mixed with 60 µl of 4 mM ATP/MgCl2, and the fluorescence signal was monitored for 20 min using a 495 nm cutoff filter (KV 495, Schott). Alexa Fluor 488 was excited at 465 nm. An increase of fluorescence was observed when the duplex was separated. Control experiments included 60 µl of protein–DNA or protein–RNA mixture mixed with buffer, and DNA or RNA duplex alone mixed with ATP/MgCl2. Data were analyzed and plotted using Prism (GraphPad).
Gel-based helicase assays
The same 3’-overhang DNA as for stopped-flow/fluorescence-based unwinding assays, but lacking fluorophore and quencher moieties, was used for gel-based unwinding assays (for sequences see Supplementary Table 4). The long strand was 5’-[32P] labeled by incubating 5 µl of 20 µM oligonucleotide, 20 µl of γ[32P]-ATP (Hartmann Analytic), 3 µl 10× reaction buffer (New England Biolabs) and 2 µl T4 polynucleotide kinase (New England Biolabs) for 1 h at 37 °C. The labeled oligonucleotide was further passed through a Microspin G25 column (Sigma), PCI-extracted and annealed to the short, unlabeled complementary oligonucleotide in a 1:1 molar ratio in annealing buffer (10 mM TRIS-HCl, pH 8.5). Unwinding reactions were carried out at 30 °C. 150 nM purified recombinant ASCC3 variants were mixed with 12 nM DNA substrate in 40 mM TRIS-HCl, pH 7.5, 0.5 mM MgCl2, 187 mM NaCl, 3.75% glycerol, 0.75 mM DTT. After a 10 min incubation at 30 °C, reactions were started by addition of 5 mM ATP/MgCl2. Aliquots were withdrawn at selected time points and reactions were quenched with 50 mM TRIS-HCl, pH 7.5, 50 mM EDTA, 0.5% [w/v] SDS). For 0 min time points, all reactants except ATP/MgCl2 were added. Samples were separated on 12% non-denaturing polyacrylamide gels, DNA bands were visualized using a phosphorimager and quantified with ImageQuant software (Cytiva). The fraction of unwound DNA in each sample was calculated as Iss/(Iss + Ids), in which Iss is the intensity of the single-stranded DNA band and Ids is the intensity of the double-stranded DNA band. The fraction unwound at 0 min was subtracted from the fraction unwound for each sample.
Reporting summary
Further information on research design is available in the Nature Research Life Sciences Reporting Summary linked to this article.
Data availability
Structure factors and coordinates have been deposited in the RCSB Protein Data Bank (https://www.rcsb.org/) with accession code 6YXQ. Source data for Figs. 1a–c, 3a–c, i, j, 5c–h, 6 and Supplementary Fig. 2 are provided. Other data are available from the corresponding author upon reasonable request. Source data are provided with this paper.
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Acknowledgements
We acknowledge access to beamline BL14.1 of the BESSY II storage ring (Berlin, Germany) via the Joint Berlin MX Laboratory sponsored by Helmholtz Zentrum Berlin für Materialien und Energie, Freie Universität Berlin, Humboldt-Universität zu Berlin, Max-Delbrück Centrum, Leibniz-Institut für Molekulare Pharmakologie and Charité–Universitätsmedizin Berlin. We thank the Biological Mass Spectromety Unit (Dr. Christoph Weise) at the DFG-funded core facility BioSupraMol of Freie Universität Berlin for mass spectrometric fingerprinting. J.J. was sponsored by a fellowship from the Chinese Scholarship Council. A.J.P.B. was supported by a postdoctoral fellowship of the Alexander von Humboldt Foundation. M.C.W. acknowledges funding by the QBI-FUB Collaborative Integrative Structural Biology Initiative. M.T.B. and K.E.B. acknowledge funding by the Deutsche Forschungsgemeinschaft (BO3442/1-2 to M.T.B. and SFB860 to K.E.B.) and the University Medical Centre Göttingen.
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J.J. performed experiments with help by E.A., N.H., and A.J.P.B., except generation of stable cell lines, pulldown experiments and western blotting, which were performed by P.H. and K.E.B. J.J. and M.C.W. wrote the manuscript with input from the other authors. All authors participated in data interpretation. M.T.B. and M.C.W. coordinated the studies. M.T.B., K.E.B., and M.C.W. provided funding for the studies. Correspondence and requests for materials should be addressed to M.C.W.
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Jia, J., Absmeier, E., Holton, N. et al. The interaction of DNA repair factors ASCC2 and ASCC3 is affected by somatic cancer mutations. Nat Commun 11, 5535 (2020). https://doi.org/10.1038/s41467-020-19221-x
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DOI: https://doi.org/10.1038/s41467-020-19221-x
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