Fig. 1: Overall structure of CV30 Fab in complex with SARS-CoV-2 RBD and kinetics of glCV30.

a Structure is shown in cartoon with surface representation shown in transparency. CV30 heavy chain is shown in dark blue and light chain in light blue. RBD is shown in pink. b Sequence alignment of CV30 heavy and light chains with germline genes. Black circles underneath the sequence indicate residues that interact with the RBD. c Details of the interactions of the heavy (left) and light (right) chains with the RBD. Complementary determining regions (CDRs) are labeled and colored as shown. Residues that interacts are shown as sticks and hydrogen bonds are shown in dotted lines. d Kinetics of glCV30 binding to RBD measured by BLI. Experimental data are shown in red with fitted curve shown in black. Dashed line represents the transition from association to dissociation phase. e CV30 and glCV30 neutralization of SARS-CoV-2 pseudovirus. AMMO1 is an EBV-specific antibody included as a control. f. CV30 and glCV30 neutralization of SARS-CoV-2 live virus. e, f Data points represent the mean of duplicates. Each experiment was repeated two times independently with similar results. d, e, f. Source data are provided as a Source Data file.