Fig. 1: Reversing EMT by inducing miR-200c expression changes organoid morphology. | Nature Communications

Fig. 1: Reversing EMT by inducing miR-200c expression changes organoid morphology.

From: Morphological screening of mesenchymal mammary tumor organoids to identify drugs that reverse epithelial-mesenchymal transition

Fig. 1

a Schematic diagram of 3D organoid cultures in regular culture format. b Morphology of vehicle- or dox-treated T11 and T12 organoids by phase-contrast microscopy. Representative images of independent biological duplicates (n = 10). Scale bar: 100 μm. c IF staining of Keratin 8 in vehicle- or dox-treated T11 organoid sections. Representative images of independent biological triplicates. Scale bar: 20 μm. d F-actin staining of vehicle- or dox-treated T11 whole-mount organoids. Representative images of independent biological triplicates. Scale bar: 50 μm. e Z-cad reporter imaging in vehicle- or dox-treated T11 whole-mount organoids. EMT cells would be green and MET cells would be red based on the design of the reporter system. Representative images of independent biological triplicates. Scale bar: 50 μm. f Immunoblotting assay of EMT markers in vehicle- or dox-treated T11 and T12 organoids. Representative images of independent biological triplicates.

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