Fig. 4 | npj Aging and Mechanisms of Disease

Fig. 4

From: Identification of transcription factors that promote the differentiation of human pluripotent stem cells into lacrimal gland epithelium-like cells

Fig. 4

The effect of overexpression of the combination of PAX6, FOXC1, and SIX1. a Schematic representation of mRNA transfection procedure for PAX6, FOXC1, and SIX1. b Phase-contrast images of hESCs transfected with a combination of three TFs: PAX6, FOXC1, and SIX1 at Day 2 (left) and Day 5 (center), and an enlarged image of the boxed area in the center panel (right). Scale bar 100 µm. c The expression profiles of representative markers after PAX6, FOXC1, and SIX1 induction in hESCs. Quantitative PCR analysis of total RNA extracts at Day 5, or the control cells also grown for the same length of time (5 days) as the experimental cells. Error bars represent mean ± SD of three samples. d Immunohistochemical analysis of differentiated cells with antibodies against BARX2, KRT15, AQP5, and LTF at day 5. The control cells also grown for the same length of time (5 days) as the experimental cells. Scale bar 100 µm

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