Table 1 Top 20 most abundant OTUs in the developed biofilms at TMPs of 31 and 50 kPa under the low and high OLR conditions

From: Architecture, component, and microbiome of biofilm involved in the fouling of membrane bioreactors

OTU ID	Closest relative		Accession No.	Identity (%)	Relative abundance (%)	Fold change (relative to)
	Class	Species				10 kPa	Activated sludge
Low OLR conditions
20392	γ-proteobacteria	Alishewanella agri	KP282808	100	33.07	2.0**	1.1
6262	γ-proteobacteria	Dokdonella immobilis	NR108377	99	9.26	0.7	1.1
16852	Flavobacteriia	Flavobacterium cucumis	AB682419	99	4.92	2.5**	0.4**
21792	γ-proteobacteria	Luteimonas marina	LM643747	100	2.90	0.7**	1.0
10052	α-proteobacteria	Brevundimonas aurantiaca	KC855474	99	1.93	0.6	3.4
9255	γ-proteobacteria	Alishewanella agri	KP282808	97	1.74	1.6	1.0
22321	γ-proteobacteria	Pseudoxanthomonas mexicana	KM210271	99	1.36	1.0	0.6
8953	α-proteobacteria	Gemmobacter megaterium	NR125598	100	1.07	0.2*	0.7
20395	γ-proteobacteria	Aquimonas voraii	KM199274	99	1.02	0.5**	4.6
25035	γ-proteobacteria	Pseudoxanthomonas daejeonensis	JQ342923	97	0.97	0.3**	1.4
17206	γ-proteobacteria	Pseudomonas mendocina	LN835456	96	0.95	8.6	58.0
9157	Flavobacteriia	Fluviicola hefeinensis	JX077130	99	0.89	6.2**	29.6
24357	γ-proteobacteria	Alishewanella agri	KP282808	97	0.85	2.0	1.1
19777	Bacteroidia	Marinifilum fragile	NR044597	89	0.76	0.4**	0.5**
30089	Sphingobacteriia	Taibaiella koreensis	LN613111	89	0.74	0.7**	0.3*
27150	β-proteobacteria	Albidiferax ferrireducens	KC855480	100	0.73	4.4**	1.3
17681	α-proteobacteria	Brevundimonas terrae	KF818660	100	0.73	1.6	5.3
16298	γ-proteobacteria	Alishewanella agri	KP282808	96	0.71	1.5	1.2
19309	γ-proteobacteria	Dokdonella immobilis	NR108377	97	0.69	1.5	1.1
5351	δ-proteobacteria	Klebsiella pneumoniae	KP941758	85	0.57	0.4**	1.6**
High OLR conditions
17206	γ-proteobacteria	Pseudomonas mendocina	LN835456	96	42.15	0.6**	0.8*
25035	γ-proteobacteria	Pseudoxanthomonas daejeonensis	JQ342923	97	4.81	5.9**	3.1**
8864	Sphingobacteriia	Lewinella nigricans	NR115013	89	4.62	1.2	0.8
30480	δ-proteobacteria	Pelobacter acidigallici	NR026154	86	4.22	43.8**	38.7**
20392	γ-proteobacteria	Alishewanella agri	KP282808	100	4.11	9.7**	4.9**
1879	Sphingobacteriia	Parapedobacter koreensis	NR043933	96	2.73	1.5**	0.3**
17375	γ-proteobacteria	Pseudomonas tuomuerensis	KP973997	96	2.17	1.0	0.9
28892	Actinobacteria	Naumannella halotolerans	KP798854	82	1.81	21.8*	17.1*
25515	Flavobacteriia	Imtechella halotolerans	NR117181	94	1.75	17.9**	4.8**
6262	γ-proteobacteria	Dokdonella immobilis	NR108377	99	1.68	10.3**	6.6**
13461	α-proteobacteria	Plastorhodobacter daqingensis	KF312715	99	1.67	0.3**	0.2*
15507	γ-proteobacteria	Stenotrophomonas acidaminiphila	LN848748	100	0.86	2.6**	2.3**
15641	δ-proteobacteria	Bdellovibrio exovorus	NR102876	100	0.81	8.7^a	494.3
27770	α-proteobacteria	Pelagibacterium halotolerans	KJ939460	99	0.77	2.2**	0.9
29369	γ-proteobacteria	Pseudomonas mendocina	LN835456	94	0.67	1.5	1.6
8953	α-proteobacteria	Gemmobacter megaterium	NR125598	100	0.61	5.0**	5.4**
20395	γ-proteobacteria	Aquimonas voraii	KM199274	99	0.55	13.9**	3.2**
10305	γ-proteobacteria	Gemmobacter aquatilis	KM220014	100	0.52	2.3**	2.1**
29195	α-proteobacteria	Paracoccus pantotrophus	KT715779	97	0.52	2.9**	1.4
20393	α-proteobacteria	Falsirhodobacter deserti	KF268394	98	0.51	0.9	0.4

^* P < 0.1, ^** P < 0.05
^aNo sequence was detected in the 10 kPa TMP biofilm microbiomes, and the value indicates fold changes related to the 30 kPa TMP biofilm microbiomes

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Table 1 Top 20 most abundant OTUs in the developed biofilms at TMPs of 31 and 50 kPa under the low and high OLR conditions

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