Fig. 4
CEACAM5 overproduction downregulates expression of mesenchymal markers and impairs TGF-β signaling and impairs TGF-β signaling. a BC3_A2 cells engineered to overproduce CEACAM5 or GFP were cultured in the presence or absence of 1 ng/ml TGF-β for the indicated time periods. Cellular lysates were subjected to Western blotting for the indicated proteins. b, c Histogram shows the fold change (log scale) in phosphorylation status of p38 (b) or Smad2/3 (c) compared with that at time point 0 for cells expressing GFP. Error bars represent SEM of at least three independent experiments. d BC3_A2 cells expressing GFP or CEACAM5 were cultured in the presence or absence of 1 ng/ml TGF-β for the indicated time periods. Cellular lysates were subjected to Western blotting for the indicated proteins. All results are representative of at least three independent experiments. See also Supplementary Figs. 8 and 9
