Extended Data Fig. 8: Dilution of DYRK3 substrates during G2-to-M transition.
From: Kinase-controlled phase transition of membraneless organelles in mitosis
a, Top, time-lapse images of a cell (single Z-stack) expressing EGFP–3×NLS during G2-to-M transition. Bottom, changes in mean nuclear intensity of EGFP–3×NLS during G2-to-M transition. Data are from eleven individual cells. b, Top, time-lapse images of a cell (single Z-stack) expressing pmScarlet–NES (nuclear export signal) during the G2-to-M transition. Bottom, changes in mean cytoplasmic intensity of pmScarlet–NES during the G2-to-M transition. Data are from eight individual cells. c, Top, time-lapse images of a cell (single Z-stack) expressing EGFP–DYRK3(WT) during G2-to-M transition. Bottom, changes in mean cytoplasmic intensity of EGFP–DYRK3(WT) during G2-to-M transition. Data are from nine individual cells. d, Top, Time-lapse images of a cell (single Z-stack) expressing EGFP-PCM1(1-1468) during G2-to-M transition. Bottom, changes in mean cytoplasmic intensity of EGFP-PCM1(1-1468) during G2-to-M transition. Data are from 11 individual cells. a–d, The lines (background) show mean nuclear intensity for individual cells. Data are mean ± s.d. Time point (0 min) refers to nuclear envelope breakdown. e, Overexpressed mCherry–SRRM1 forms mitotic granules which recruit endogenous splicing proteins. f, Time-lapse images (single Z-stack) show fusion of mitotic mCherry–SRRM1 granules in mitotic cells. g, Left, FRAP analysis of interphase and mitotic mCherry–SRRM1 granules in the presence and absence of the GSK-626616 inhibitor (1 μM). Data are mean ± s.d. Right, FRAP recovery of mitotic mCherry–SRRM1 granule. h, Cells arrested in mitosis show formation of splicing granules upon GSK-626616 treatment (1 μM, indicated times). Data and images are representative of at least three independent experiments. Scale bars, 10 μm.
