Extended Data Fig. 9: Growth factor induction of laminin, comparison of propriospinal and serotonin axons, and locomotor evaluations of rats after SCI without and with treatments.
From: Required growth facilitators propel axon regeneration across complete spinal cord injury
a, b, Survey images show laminin 1 IHC in tiled mosaic scans of horizontal sections from representative rats. b, Top, mean ± s.e.m. quantification of laminin IHC in rats as per cent area per linear µm3, (n = 4 rats per group, dark coloured lines = means, lighter coloured shaded areas = s.e.m., colours indicate experimental groups as shown in graph below). Bottom, total laminin in rats summarized as mean ± s.e.m. area under the curve as calculated from graph above. (ns non-significant, **P < 0.005, one-way ANOVA/Bonferroni, F (2, 9) = 15.04). c, Multiple channel fluorescent images show RFP-labelled propriospinal axons and immunohistochemically stained serotonin (5HT) axons in rats after SCI + AAV-OIC + 2D + FGF + EGF + GDNF. The two survey images on the left show the same field with different filters. Note in the survey images on the left, and in the higher magnification image in centre, that RFP-labelled propriospinal axons regrow robustly past the astrocyte scar proximal border and through the non-neural lesion core. By contrast, 5HT axons did not regrow into or through the lesion core. The image on the right shows an orthogonal 3D confocal detail from an area proximal to the SCI lesion, demonstrating a complete lack of overlap of RFP labelling and 5HT immunohistochemistry, indicating that RFP tracing did not label 5HT axons of passage. d, Open field hindlimb locomotor score at various times after SCI in rats assessed using a 6-point scale in which 5 is normal walking and 0 is no movement of any kind. Data are mean ± s.e.m., n = 6 per rats group.
