Extended Data Fig. 7: Liver-specific Atg5-deleted hosts present liver-cell enlargement, increased serum ARG1 and decreased serum arginine.
From: Autophagy maintains tumour growth through circulating arginine
a, Experimental design to induce liver-specific deletion of Atg5. Atg5flox/flox mice were injected in the tail vein with AAV–TBG–GFP or AAV–TBG–iCre at 8 to 10 weeks of age to delete Atg5 in the liver and create Atg5+/+ hosts and hosts with liver-specific deletion of Atg5, respectively. b, Western blotting showing expression of Atg5 in the livers, brains and kidneys of Atg5+/+ hosts and hosts with liver-specific deletion of Atg5 (n = 6 each). *P < 0.05 compared to Atg5+/+ hosts. Actin was used as a loading control c, Haematoxylin and eosin tissue staining from Atg5+/+ hosts and hosts with liver-specific deletion of Atg5 (n = 6 each). d, Western blotting showing expression of ARG1 in serum from Atg5+/+ hosts and hosts with liver-specific deletion of Atg5 (n = 6 each). *P < 0.05 compared to Atg5+/+ hosts. Transferrin was used as a loading control. e, Levels of arginine, ornithine and citrulline in serum in Atg5+/+ hosts and hosts with liver-specific deletion of Atg5 (n = 6 each), obtained by LC–MS. Data are mean ± s.e.m. ***P < 0.001, ****P < 0.0001.
