Extended Data Fig. 8: Inflammation resolution and coagulation stay unaffected during fascia blocking. | Nature

Extended Data Fig. 8: Inflammation resolution and coagulation stay unaffected during fascia blocking.

From: Patch repair of deep wounds by mobilized fascia

Extended Data Fig. 8

a, Sham (left) or ePTFE-implanted (right) wounds at 7 (top) or 63 dpw (bottom) immunolabelled for collagen I, III and VI. Representative images of three biological replicates. b–i, Immunolabellings (left) and fractions (right) of immune cells (PTPRC+; b), neutrophils (LY6G+; c), T cells (CD3+; d), NK cells (NCR1+; e), B cells (CD19+; f), macrophages and monocytes (MOMA2+; g) and cells expressing the pro- and anti-inflammatory cytokines (IL12 (h) and IL4 (i)). Mean with SEM, n = 3 images analysed from 3 biological replicates. Unpaired two-tailed t-test, 95% CI (b). One-way ANOVA, Tukey’s test, 95% CI (c-i). j, Activated platelets (SELP) in 7 dpw sham and ePTFE-implanted wounds. Representative images of 3 biological replicates. k, Mean grey value of SELP signal. Data are mean ± s.e.m.; n = 3 images analysed from 3 biological replicates. Two-tailed Student’s t-test, 95% CI. Dotted lines delimit the wound area. Scale bars, 200 μm (main images) and 100 μm (magnified insets).

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