Extended Data Fig. 9: Food-search behaviour in knockout mice and analysis of Npy5r expression.
From: Hunger enhances food-odour attraction through a neuropeptide Y spotlight
a, Latency to discover a food pellet buried in bedding was timed in the indicated fasted (left) and fed (right) mice. n = 3 male mice and 3 female mice, data are mean ± s.e.m. **P < 0.01, *P < 0.05, Kruskal–Wallis test with Dunn’s multiple comparison. b, RNA in situ hybridization using Npy5r anti-sense and sense probes in coronal brain cryosections with PVT (left, dashed lines) and piriform cortex. Scale bar, 500 μm. c, AGRP neuron projections to the PVT were visualized by immunohistochemistry for tdTomato in Agrp-ires-dre mice injected in the arcuate nucleus with AAV-DIO-tdTomato. Scale bar, 500 μm. Images in b and c are representative of three independent experiments involving different mice.
