Fig. 2: SARS-CoV-2 RBD-specific B cell memory.
From: Naturally enhanced neutralizing breadth against SARS-CoV-2 one year after infection
a, Number of antigen-binding memory B cells per 2 × 106 B cells (Extended Data Fig. 5b, c) obtained at 1.3, 6.2 and 12 months after infection from 40 randomly selected individuals (vaccinated, n = 20; non-vaccinated, n = 20). Each dot represents one individual. Red horizontal bars indicate geometric mean values. Two-sided Kruskal–Wallis test with subsequent Dunn’s multiple comparisons. WT, wild type. b, The distribution of antibody sequences from 6 individuals 1.3 (top) or 6.2 (middle) or 12 (bottom) months after infection3,4. The number in the inner circle indicates the number of sequences analysed for the individual whose identifier is denoted above the circle. Pie slice size is proportional to the number of clonally related sequences. The outer black arc indicates the frequency of clonally expanded sequences detected in each participant. Coloured slices indicate persisting clones (same IGV and IGJ genes, with highly similar CDR3 sequences) found at both time points in the same participant. Grey slices indicate clones unique to the time point. White indicates sequences isolated once, and white slices indicate singlets found at both time points. c, Number of somatic nucleotide mutations (SHM) in the IGVH and IGVL genes (Supplementary Table 3) obtained after 1.3 or 6.2 or 12 months (1.3 month, n = 889; 6.2 month, n = 975; 12 month, n = 1,105 (unvaccinated, n = 417; vaccinated, n = 688)). Red horizontal bars indicate mean values. Two-sided Kruskal–Wallis test with subsequent Dunn’s multiple comparisons.
