Extended Data Fig. 6: Comparison of H9T with natural cytokines in human CD8⁺ T cells.

a, Pre-activated human CD8⁺ cells were rested and cultured with 10 nM indicated cytokines for 6 days, and TIM-3 expression was examined. Data are mean ± SEM, one-way ANOVA test with Dunnett’s correction, n = 6 donors. Data are from two independent experiments. b–d, Pre-activated human CD8⁺ T cells were rested and cultured with 10 nM of the indicated cytokines for 0, 0.5, 1, 2 and 4 h and stained with anti-pSTAT5, pAKT, and pERK. Data are mean ± SEM, n = 6 donors. Data are from two independent experiments. e–i, Pre-activated human CD8⁺ T cells were rested and cultured with 10 nM of the indicated cytokines for 24 h and cells were collected for RNA-seq. Selected genes expression (e), PCA analysis (f) and GSEA were shown, Kolmogorov–Smirnov test. Data are from three donors. j–l, Pre-activated human CD8⁺ T cells were rested and cultured with 10 nM of the indicated cytokines for 6 days, and stained with anti-CD27, CCR7 and Granzyme B antibodies. Data are mean ± SEM, one-way ANOVA test with Dunnett’s correction, n = 6 donors. Data are from two independent experiments. m, Pre-activated human CD8⁺ cells were rested and cultured with 10 nM of the indicated cytokines for 6 days, followed by TMRM analysis. Data are mean ± SEM, one-way ANOVA test with Dunnett’s correction, n = 5 donors. Data are from two independent experiments. n, Pre-activated human CD8⁺ T cells were rested and cultured with 1 nM IL-2 alone or in the presence of 1, 10 and 100 nM H9T or IL-15. TIM-3 expression was analysed by flow cytometry after 2 days. Data are mean ± SEM, one-way ANOVA test with Dunnett’s correction, n = 5 donors. Data are from two independent experiments

Source data.