Fig. 1: Overview of the experimental design and data processing.
From: Benchmarking single-cell RNA-sequencing protocols for cell atlas projects
The reference sample consists of human PBMCs (60%), and HEK293T (6%), mouse colon (30%), NIH3T3 (3%) and dog MDCK cells (1%). The sample was prepared in one single batch, cryopreserved and sequenced by 13 different sc/snRNA-seq methods. Sequences were uniformly mapped to a joint human, mouse and canine reference, and then separately to produce gene expression counts for each sequencing method.
