Extended Data Fig. 4: Improved detection of protein-coding and non-coding RNAs with Smart-seq3.
From: Single-cell RNA counting at allele and isoform resolution using Smart-seq3
(a) Variants of Smart-seq3 reactions (n = 16 HEK293FT cells per condition) show improved detection of protein coding RNAs and also other classes of RNAs, including poly-A + lincRNAs, antisense RNAs, processed pseudogenes, processed transcripts and snoRNAs, compared to Smart-seq2 (n = 48 HEK293FT cells) and earlier experimental versions of Smart-seq2 with UMIs (here called “intermediate”) (n = 32 HEK293FT cells). (b) Shows genes of similar RNA classes detected by UMI containing reads in Smart-seq2 protocols using UMIs (here called “intermediate”) (n = 32 cells) and Smart-seq3 variants (n = 16 cells per condition).
