Supplementary Figure 8: K562 control experiments for AluJb-LIN28B candidate validation.

a, Promoter-luciferase (n = 3 independent experiments) results illustrating transcriptional activity of various TE arrangements in K562. Welch’s t-test was performed against reverse complement AluJb sequence (limited activity). b, Luciferase assays (n = 3 independent experiments) for mutagenized transcription factor motifs in K562. Relative luciferase activty for mutagenized vectors were compared to wild-type AluJb-P. c, Genotypes of K562 CRISPR KO clones for AluJb-P and LIN28BP deletions. Genotype check was repeated twice with similar results. d, Cropped Western blot for LIN28B in K562 CRISPR KO clones. K562 also expresses a smaller isoform of LIN28B that is not present in H1299 and H838. This experiment was repeated twice with similar results. e, Relative let-7a, let-7b and let-7g miRNA levels compared to wild-type in CRISPR-knockout clones of K562 as measured by qPCR (n = 3 independent experiments). f, CCK-8 growth assay measuring cell growth rate of K562 WT and CRISPR clones (n = 3 independent experiments). a,b, P-values were calculated using two-tailed Welch t-test. a,b,e,f, All data are represented as means ± SE.