Supplementary Figure 7: Treg cells derived from IL2 and IL4 exhibit distinct phenotypes.
From: Thymic regulatory T cells arise via two distinct developmental programs
a,b) Flow plots of the indicated TregP cell subsets following 3 days of stimulation with the indicated cytokines. c,d) Quantification of the gMFI of CD25 or Foxp3 within mature Treg cells (CD25+Foxp3+) generated from the indicated cytokine conditions. Data was analyzed by two-sided Kruskal-Wallis test. Data represents 3 experiments, n = 9 (CD25+ TregP cells- 1 Uml–1 IL2, 1 ng/mL IL4), n = 8 (CD25+ TregP cells- 100 ng/mL IL4, 1 Uml–1 IL2 + 1 ng/mL IL4, 1 Uml–1 IL2 + 100 ng/mL IL4; Foxp3lo TregP cells- 1 Uml–1 IL2, 1 Uml–1 IL2 + 1 ng/mL IL4, 1 Uml–1 IL2 + 100 ng/mL IL4), n = 7 (Foxp3lo TregP cells- 1 ng/mL IL4, 100 ng/mL IL4) or 1 experiment, n = 3 (CD25+ TregP cells- 0.5 ng/mL IL4, 1 Uml–1 IL2 + 0.5 ng/mL IL4; Foxp3lo TregP cells- 1 Uml–1 IL2 + 0.5 ng/mL IL4), n = 2 (Foxp3lo TregP cells- 0.5 ng/mL IL4) replicates. Bars represent mean ± SD. *P<0.05; **P<0.005; ***P<0.0001.
