Supplementary Fig. 6: Bhlhe40 is required for normal proliferation of thioglycollate-elicited macrophages during type 2 immunity. | Nature Immunology

Supplementary Fig. 6: Bhlhe40 is required for normal proliferation of thioglycollate-elicited macrophages during type 2 immunity.

From: Bhlhe40 mediates tissue-specific control of macrophage proliferation in homeostasis and type 2 immunity

Supplementary Fig. 6

a, Flow cytometry of BrdU incorporation by LPMs and Thio-elicited macrophages from Bhlhe40+/+ and Bhlhe40-/- mice treated with PBS, IL-4c, thioglycollate (Thio), or Thio and IL-4c as in Fig. 5j. b,c, Frequency of pHH3+ LPMs and Thio-elicited macrophages (b) (pooled from 2 experiments, n = 3 PBS-treated Bhlhe40+/+; 4 PBS-treated Bhlhe40-/-; 4 IL-4c-treated and Thio-treated Bhlhe40+/+ and Bhlhe40-/-; 6 Thio and IL-4c-treated Bhlhe40+/+ and Bhlhe40-/-) and RELMα+ LPMs and Thio-elicited macrophages (c) (pooled as in b) from Bhlhe40+/+ and Bhlhe40-/- mice treated with PBS, IL-4c, thioglycollate (Thio), or Thio and IL-4c. Data in b,c are mean ± s.e.m; each symbol represents an individual mouse (b,c). *P ≤ 0.05; ***P < 0.001, unpaired two-sided Student’s t-test.

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