Extended Data Fig. 4: Peripherally induced CNS Trm cells are resistant to systemic depletion.
a,b, Thy1.1 eGFP+ OT-I T-cells were transferred into naïve mice that were DC-OVA-rLM-OVA prime boosted. At a memory time point, mice were control treated (PBS) or treated with 2, 5, or 10 μg of anti-Thy1.1 Ab IP. One week after depletion, proportions and phenotype of OT-I cells were determined. Representative flow plots gated on live CD8+ cells from Spleen, PBL and IV+ or IV– brain showing proportions of OT-I cells (a) and gMFI of CD69 gated on IV– OT-I cells (b). Graphs show the mean +/- standard deviation with each dot representing an individual mouse. Data are representative of 2 individual experiments with 3 mice per group. Statistical significance was determined by One-way ANOVA with Tukey’s multiple comparisons test across all the groups using graphpad prism with p values (top to bottom) *** p=0.0005, *** p=0.0004, and * p=0.0144. c, Thy1.1 eGFP+ OT-I T-cells were transferred into naïve mice that were DC-OVA-rLM-OVA prime boosted. After memory formation, mice were control treated (PBS) or treated with 2 μg of anti-Thy1.1 Ab IP. One week after depletion, OT-I cells in the CNS were imaged and maximum speed was determined. Graph depicts mean with each dot representing an individual OT-I cell from 71 cells from 12 individual movies (PBS), and 103 cells from 16 individual movies (2 μg). Statistical significance was determined by two-tailed Mann Whitney test using graphpad prism with **** p<0.0001.
