Supplementary Figure 6: Fluorescence microscopy of FACS-sorted in vitro conjugations.
From: Metagenomic engineering of the mammalian gut microbiome in situ
Overlays of bright-field, GFP, and mCherry channels are shown alongside GFP and mCherry channels. Q3 populations from unmodified fecal bacteria are negative for both GFP and mCherry, whereas Q4 populations from aerobic overnight in vitro conjugations of vector libraries pGT-L3 and pGT-L7 show enrichment of GFP-expressing cells, as well as some donor cells (mCherry+GFP–), which were eliminated in downstream sequencing analyses. This experiment was performed independently three times, with similar results.
