Extended Data Fig. 1: Structural features visible in optical sections with THG that facilitate navigation through the brain. | Nature Methods

Extended Data Fig. 1: Structural features visible in optical sections with THG that facilitate navigation through the brain.

From: Deep three-photon imaging of the brain in intact adult zebrafish

Extended Data Fig. 1

A. Telencephalic region, showing from left to right: the skin surface features; the transition into the brain as shadows of neurons become visible; and deeper in the telencephalon where the shadows of the neurons clustered at the midline are evident, as are scattered neurons throughout the section. B. Optic Tectum, showing from left to right: axon bundles on the surface of the tectum just below the skin and skull, with scales on the skin visible; deeper axon bundles in fiber layers of the tectum; densely packed shadows of cell bodies deep in the tectum; a major blood vessel lying adjacent to the ventricle deep in tectum. OT N= 16, Tel N=12.

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