Supplementary Figure 6: HIV-1 virions treated with the protease inhibitor saquanivir do not induce CD4 and CXCR4 oligomerization nor positive Bcc.
From: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction
a–c, COS-7 cells co-expressing CD4-mOrange and CXCR4-mTFP1 were imaged before and after exposure to HIVHXB2 virions (left panels). As shown before (Supplementary Fig. 3), these COS-7 cells show an overall increase in the CD4 oligomeric state (left panel in a) and a significant increase in positive Bcc (left panel in c). The 0.05 level for one-way ANOVA shows that the population means are significantly different. When treating the cells with 320 nM SQV (right panels), no significant increase in CD4 oligomerization or positive Bcc was observed (left panels in a and c). The 0.05 level for one-way ANOVA shows that the population means are not significantly different. In all cases, n = 10.
