Supplementary Figure 8: HIV-1 infection assays validate the use of COS-7 cells expressing labelled CD4 and co-receptors. | Nature Structural & Molecular Biology

Supplementary Figure 8: HIV-1 infection assays validate the use of COS-7 cells expressing labelled CD4 and co-receptors.

From: A dynamic three-step mechanism drives the HIV-1 pre-fusion reaction

Supplementary Figure 8

COS-7 cells (single images, left column; tiled images, right column) were exposed to HIVHXB2 virions (first row), HIVHXB2 virions treated with 350 nM saquanivir (SQV) (second row), HIVVSV-G virions (third row), no-Env HIV-1 virions (fourth row) and HIVJR-FL virions (fifth row). Expression of Gag-iCherry after 24 h only for infection with HXB2-, VSV-G- and JR-FL-decorated HIV-1 virions validates the use of these reporter cells. Scale bars, 1 μm (left column) and 10 μm (right column).

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