Supplementary Figure 2: Different ratios of DCs to naive CD8+ T cells. | Nature Protocols

Supplementary Figure 2: Different ratios of DCs to naive CD8+ T cells.

From: Induction of neoantigen-reactive T cells from healthy donors

Supplementary Figure 2

M2-minigene-electroporated DCs were cocultured with T cells at DC:T cell ratios of 1:8, 1:4, 1:2 and 1:1 (n = 4 donors, 3 cultures/donor), and cultures were analyzed for the presence of neo-1, -2, -3, -4 and -5 pMHC-multimer-reactive populations on day 12. Each dot represents the percentage of pMHC-multimer-positive cells among CD8+ cells identified by flow cytometry.

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