Figure 8
From: Metabolomic and Proteomic Analysis of Maize Embryonic Callus induced from immature embryo
Expression pattern of genes potentially regulated by histone deacetylase. (a) Heatmap of proteins in the 18R blue module that show a significantly negative correlation with HDT2 (GRMZM2G100146) and HDT3 (GRMZM2G159032). No. of GCC box is the number of “GCCGCC” sequences in the promoter (3000 bp upstream of ATG). The “GCC box” was reported to be bound by ERF to regulate gene expression. ERF can directly interact with histone deacetylases to further function. (b) Heatmap of transcription changes of potential downstream genes of ERF and histone deacetylase at the 3 stages of 18R. (c) Relative expression level of HDT2 and HDT3 are shown as means ± SD (n = 3) during callus formation of 18R. *P < 0.05 (Student’s t test) for differences between control and each stage.
