Table 4 Polymorphisms in pfK13.

From: High throughput resistance profiling of Plasmodium falciparum infections based on custom dual indexing and Illumina next generation sequencing-technology

Base position

Codon position

Reference base

Alternative base

Reference codon

Alternative codon

Reference freq.

Alternative freq.

Mixed freq.

Non-synonymous K13 polymorphisms

65

22

G

A

R

K

99.7%

0.3%

0.0%

252

84

A

G

L

K

99.7%

0.3%

 

335

112

G

A

G

E

99.7%

0.9%

0.6%

340

114

A

C

L

Q

99.1%

1.3%

0.3%

445

149

A

T

T

S

99.4%

0.6%

 

566

189

A

C

L

T

53.8%

57.9%

11.6%

567

189

A

T

L

N

98.1%

3.5%

1.6%

820

274

C

T

H

Y

99.7%

0.3%

 

1732

578

G

T

A

S

99.1%

1.6%

0.6%

2092

698

T

A

F

I

99.7%

0.3%

 

Synonymous K13 polymorphisms

57

19

G

A

T

T

99.7%

0.3%

 

321

107

T

C

S

S

99.7%

0.3%

 

417

139

T

C

N

N

99.4%

0.9%

0.3%

1407

469

C

T

C

C

99.1%

1.9%

0.9%

1434

478

C

G

T

T

99.7%

0.3%

 

1881

627

T

A

A

A

99.7%

0.3%

 

2070

690

C

A

G

G

99.7%

0.3%

 
  1. The table describes the various polymorphisms found in pfK13 for the 318 resistance-profiled samples, and the frequencies in which the reference and alternative base were found, as well as the frequencies of mixed infections. SNP positions written in italic indicate that they are located in the propeller region of pfK13. Note that mixed infections were counted in both the reference and alternative frequencies.