Figure 1
From: Optofluidic device for the quantification of circulating tumor cells in breast cancer
Optofluidic Chip. (A) SEM image of the optofluidic chip. (B and C) Confinement of the sample at the central region of the channel. A blue light path (C) is visible only when a cell intersects the pumping beam. Absorption and emission properties of the dyes used to label the antibodies and their performance on the cell membrane. (D) Absorption and emission profiles of anti-HER2-FITC (green) and anti-EpCAM-PerCP/Cy5.5 (red) antibodies. (E) Laser scanning confocal microscopy images of AU-565 cells stained with anti-HER2-FITC conjugated (green) and anti-EpCAM-PerCP/Cy5.5 conjugated (red) antibodies. Nuclei were stained with DAPI (blue). Scale bar 10 μm.
